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Brilliant Violet 421™ anti-human GPR183 (EBI2) Antibody

Pricing & Availability
Clone
SA313E4 (See other available formats)
Regulatory Status
RUO
Other Names
EBI2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA313E4_BV421_GPR183_Antibody_1_122116
Human peripheral blood lymphocytes were stained with CD19 PE and GPR183 (clone SA313E4) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • SA313E4_BV421_GPR183_Antibody_1_122116
    Human peripheral blood lymphocytes were stained with CD19 PE and GPR183 (clone SA313E4) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • SA313E4_BV421_GPR183_Antibody_2_122116
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
368909 25 tests £133
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368910 100 tests £291
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Description

GPR183, also known as EBI2, is a member of the rhodopsin-like subfamily of 7TM receptors, which forms homodimers and heterodimers when it associates with CXCR5. GPR183 is expressed by Naive B cells, subset of T cells, monocytes and macrophages, and is highly upregulated by Epstein–Barr virus infection. GPR183 regulates the B cell trafficking within lymphoid follicles in response to 7α, 25-dihydroxycholesterol.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human GPR183-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
RRID
AB_2632943 (BioLegend Cat. No. 368909)
AB_2632943 (BioLegend Cat. No. 368910)

Antigen Details

Structure
Member of the rhodopsin-like subfamily of 7TM receptors and forms homo- and heterodimers.
Distribution

Naive B cells, subset of T cells, monocytes and macrophages

Function
Chemotactic receptor that regulates B cell trafficking within lymphoid follicles.
Ligand/Receptor
7α, 25-dihydroxycholesterol
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors, Steroid Receptors/Nuclear Receptors
Antigen References

1. Preuss I, et al. 2014. Biochem. Biophys. Res. Commun. 446:663.
2. Barroso R, et al. 2012. FASEB J. 26:4841.
3. Hannedouche S, et al. 2011. Nature. 475:524.
4. Benned-Jensen T, et al. 2011. J. Biol. Chem. 286:29292.

Gene ID
1880 View all products for this Gene ID
UniProt
View information about GPR183 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 12/22/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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