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Brilliant Violet 421™ anti-mouse CD134 (OX-40) Antibody

Pricing & Availability
Clone
OX-86 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF4, ACT35, OX-40
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
OX-86_BV421_112811
Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • OX-86_BV421_112811
    Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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119411 125 µL £140
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Description

CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse OX-40-CD4 chimeric protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone OX-86 has been reported to act as an agonist and stimulate OX-40.

Application References

(PubMed link indicates BioLegend citation)
  1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC)
  2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
  3. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  2. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
RRID
AB_10962569 (BioLegend Cat. No. 119411)

Antigen Details

Structure
TNF receptor superfamily, 50 kD
Distribution

Activated CD4+ and CD8+ T cells, activated B cells.

Function
Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
Ligand/Receptor
OX-40 ligand
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695.
2. Weinberg AD, et al. 1999. J. Immunol. 162:1818.
3. Akira H, et al. 1999. J. Immunol. 162:7058.
4. Pippig SD, et al. 1999. J. Immunol. 163:6520.
5. Higgins LM, et al. 1999. J. Immunol. 162:486.

Gene ID
22163 View all products for this Gene ID
UniProt
View information about CD134 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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