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Brilliant Violet 421™ anti-mouse CD282 (TLR2) Antibody

Pricing & Availability
Clone
CB225 (See other available formats)
Regulatory Status
RUO
Other Names
TLR-2, mTLR2, Ly105
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
CB225_BV-421_CD282_FC_041524
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with anti-mouse F4/80 (clone BM8) APC and anti-mouse CD282 (TLR2) (clone CB225) Brilliant Violet 421™ (left), or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • CB225_BV-421_CD282_FC_041524
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with anti-mouse F4/80 (clone BM8) APC and anti-mouse CD282 (TLR2) (clone CB225) Brilliant Violet 421™ (left), or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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148605 50 µg £165
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Description

CD282, also known as TLR2, is an 89 kD, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular region and one toll/IL-1R (TIR) domain in the intracellular region. TLR2 is mainly expressed on monocytes, macrophages, dendritic cells, granulocytes, and endothelial and epithelial cells. TLR2 associates with TLR1 and TLR6, and mediates the innate immune response against several components of Gram-positive bacteria, such as peptidoglycan, lipoteichoic acid (LTA), lipoarabinomanan, and lipoproteins as well as different LPS from certain Gram-negative bacteria, yeast, spirochetes, and fungi.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TLR2-transfected Ba/F3 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

1. Shibata T, et al. 2012. Int. Immunol. 24:613. (FC)

RRID
AB_3106173 (BioLegend Cat. No. 148605)

Antigen Details

Structure
89 kD type I transmembrane protein with 14 leucine-rich repeats (LRR) on its extracellular region and one toll/IL-1R (TIR) domain on its intracellular region.
Distribution

Monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, epithelial cells.

Function
Activates innate host defense against a diverse set of pathogens.
Interaction
TLR1, TLR6.
Ligand/Receptor
Microbial lipoproteins, peptidoglycans, lipoteichoic acid.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Du B, et al. 2013. J. Biol. Chem. 288:15131.
2. Zivkovic A, et al. 2011. J. Immunol. 186:1608. 
3. Okun E, et al. 2010. J. Neurochem. 114:462. 
4. Kim S, et al. 2009. Nature. 457:102.

Gene ID
24088 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04/15/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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