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Brilliant Violet 421™ anti-mouse CX3CR1 Antibody

Pricing & Availability
Clone
SA011F11 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA011F11_BV421_CX3CR1_Antibody_FC_1_050515
C57BL/6 mouse splenocytes were stained with CD11b APC and CX3CR1 (clone SA011F11) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • SA011F11_BV421_CX3CR1_Antibody_FC_1_050515
    C57BL/6 mouse splenocytes were stained with CD11b APC and CX3CR1 (clone SA011F11) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • SA011F11_BV421_CX3CR1_Antibody_FC_2_050515
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
149023 50 µg £200
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Description

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse CX3CR1-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

View more applications data for a different format of this clone in our Scientific Poster Library.

Product Citations
  1. De Biase LM et al. 2017. Neuron. 95(2):341-356 . PubMed
  2. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  3. De Schepper S, et al. 2023. Nat Neurosci. 26:406. PubMed
  4. Lu QK, et al. 2022. Acta Pharmacol Sin. 43:376. PubMed
  5. Leonardi I, et al. 2022. Cell. 185:831. PubMed
  6. Boroumand P, et al. 2022. Elife. 11:. PubMed
  7. Chen R, et al. 2023. Acta Pharm Sin B. 13:128. PubMed
  8. Takimoto Y, et al. 2023. iScience. 26:106220. PubMed
  9. Valencia–Hernandez AM, et al. 2020. Cell Host & Microbe. 27(6):950-962. PubMed
  10. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  11. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  12. Christofi M, et al. 2020. Cell Mol Life Sci. . PubMed
  13. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  14. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  15. Burns JC, et al. 2021. Bio Protoc. 11:e4091. PubMed
  16. Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed
  17. Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed
  18. Zhu H, et al. 2021. Cell Metabolism. :. PubMed
  19. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  20. Burns JC, et al. 2020. eLife. 9:00. PubMed
  21. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  22. Highton AJ, et al. 2019. Wellcome Open Res. 4:78. PubMed
  23. Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed
  24. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  25. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  26. Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
  27. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  28. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
RRID
AB_2565706 (BioLegend Cat. No. 149023)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution

Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.

Function
Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor
CX3CL1 (fractalkine, neurotactin).
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ponzetta A, et al. 2013. J. Immunol. 191:5684.
2. Jacquelin S, et al. 2013. Blood. 122:674.
3. Garcia JA, et al. 2013. J. Immunol. 191:1063.
4. Lee YS, et al. 2013. Cell. 153:413.
5. Shechter R, et al. 2013. Immunity. 38:555.

Gene ID
13051 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CX3CR1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CX3CR1 SA011F11 FC
Alexa Fluor® 647 anti-mouse CX3CR1 SA011F11 FC
PE anti-mouse CX3CR1 SA011F11 FC
APC anti-mouse CX3CR1 SA011F11 FC
PerCP/Cyanine5.5 anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 421™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 510™ anti-mouse CX3CR1 SA011F11 FC
FITC anti-mouse CX3CR1 SA011F11 FC
Alexa Fluor® 488 anti-mouse CX3CR1 SA011F11 FC
PE/Dazzle™ 594 anti-mouse CX3CR1 SA011F11 FC
PE/Cyanine7 anti-mouse CX3CR1 SA011F11 FC
Biotin anti-mouse CX3CR1 SA011F11 FC,Cell Sep - Pos
Brilliant Violet 605™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 785™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 711™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 650™ anti-mouse CX3CR1 SA011F11 FC
Alexa Fluor® 700 anti-mouse CX3CR1 SA011F11 FC
APC/Fire™ 750 anti-mouse CX3CR1 SA011F11 FC
Pacific Blue™ anti-mouse CX3CR1 SA011F11 FC
TotalSeq™-A0563 anti-mouse CX3CR1 SA011F11 PG
TotalSeq™-C0563 anti-mouse CX3CR1 SA011F11 PG
TotalSeq™-B0563 anti-mouse CX3CR1 SA011F11 PG
APC/Cyanine7 anti-mouse CX3CR1 Antibody SA011F11 FC
PE/Cyanine5 anti-mouse CX3CR1 SA011F11 FC
PE/Fire™ 700 anti-mouse CX3CR1 SA011F11 FC
APC/Fire™ 810 anti-mouse CX3CR1 SA011F11 FC
PE/Fire™ 640 anti-mouse CX3CR1 SA011F11 FC
PE/Fire™ 810 anti-mouse CX3CR1 SA011F11 FC
PerCP/Fire™ 806 anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 570™ anti-mouse CX3CR1 SA011F11 FC
PerCP/Fire™ 780 anti-mouse CX3CR1 SA011F11 FC
Spark Red™ 718 anti-mouse CX3CR1 (Flexi-Fluor™) SA011F11 FC
Spark PLUS UV395™ anti-mouse CX3CR1 SA011F11 FC
Spark Blue™ 574 anti-mouse CX3CR1 (Flexi-Fluor™) SA011F11 FC
Spark Blue™ 550 anti-mouse CX3CR1 (Flexi-Fluor™) SA011F11 FC
Go To Top Version: 2    Revision Date: 09/26/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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