- Clone
- RMG2a-62 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2a
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
407117 | 50 µg | £170 |
The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Gavin AL, et al.2006. Science 314:1936.
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
- Product Citations
-
- RRID
-
AB_2687343 (BioLegend Cat. No. 407117)
Related FAQs
- IgG2a gene is deleted in some mouse strains, which ones are they?
-
IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All IgG2a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IgG2a | RMG2a-62 | ELISA,FC |
Biotin anti-mouse IgG2a | RMG2a-62 | ELISA,FC |
FITC anti-mouse IgG2a | RMG2a-62 | FC |
PE anti-mouse IgG2a | RMG2a-62 | FC |
APC anti-mouse IgG2a | RMG2a-62 | FC |
PerCP/Cyanine5.5 anti-mouse IgG2a | RMG2a-62 | FC |
PE/Cyanine7 anti-mouse IgG2a | RMG2a-62 | FC |
Alexa Fluor® 647 anti-mouse IgG2a | RMG2a-62 | FC,IHC-P |
Brilliant Violet 421™ anti-mouse IgG2a | RMG2a-62 | FC |
Alexa Fluor® 488 anti-mouse IgG2a | RMG2a-62 | FC |
APC/Fire™ 750 anti-mouse IgG2a | RMG2a-62 | FC |
Alexa Fluor® 594 anti-mouse IgG2a | RMG2a-62 | IHC-F,IHC-P |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IgG2a
-
Biotin anti-mouse IgG2a
-
FITC anti-mouse IgG2a
-
PE anti-mouse IgG2a
-
APC anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with (filled... -
PerCP/Cyanine5.5 anti-mouse IgG2a
Human peripheral blood lymphocyte were stained with purified... -
PE/Cyanine7 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... IHC staining of Alexa Fluor® 647 anti-mouse IgG2a on formali... -
Brilliant Violet 421™ anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 488 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
APC/Fire™ 750 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 594 anti-mouse IgG2a
Human frozen tonsil section was fixed with 4% paraformaldehy... IHC staining of Alexa Fluor® 594 anti-mouse IgG2a on formali...
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