- Clone
- S16009A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prf1, Pfp, Pfn, Pfn-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S16009A_BV-421_Perforing_Antibody_092623 S16009A_BV-421_Perforing_Antibody_092623](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S16009A_BV-421_Perforing_Antibody_092623-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with anti-mouse CD49b APC. Cells were fixed, permeabilized, and stained with anti-mouse Perforin (clone S16009A) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
154319 | 50 µg | £185 |
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse perforin transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.
- RRID
-
AB_3083137 (BioLegend Cat. No. 154319)
Antigen Details
- Structure
- 70 kD Protein that oligomerizes for pore formation.
- Distribution
-
CTL, NK (Cytoplasmic Granules)
- Function
- Target cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Podack ER, et al. 1988. Immunol. Rev. 103:203.
- Konjar S, et al. 2010. Immunology. 131:257.
- Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
- Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
- Gene ID
- 18646 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Perforin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-mouse Perforin | S16009A | ICFC |
PE anti-mouse Perforin | S16009A | ICFC |
Purified anti-mouse Perforin | S16009A | ICFC |
FITC anti-mouse Perforin | S16009A | ICFC |
PE/Dazzle™ 594 anti-mouse Perforin | S16009A | ICFC |
APC/Fire™ 750 anti-mouse Perforin | S16009A | ICFC |
Pacific Blue™ anti-mouse Perforin | S16009A | ICFC |
Alexa Fluor® 647 anti-mouse Perforin | S16009A | ICFC |
Brilliant Violet 421™ anti-mouse Perforin | S16009A | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse Perforin
C57BL/6 splenocytes were stained with CD49b PE. Cells were ... -
PE anti-mouse Perforin
C57BL/6 splenocytes were stained with CD49b APC. Cells were... -
Purified anti-mouse Perforin
C57BL/6 splenocytes were fixed, permeabilized, and stained w... -
FITC anti-mouse Perforin
C57 splenocytes were stained with anti-mouse CD49b APC, fixe... -
PE/Dazzle™ 594 anti-mouse Perforin
C57BL/6 splenocytes were stained with anti-mouse CD49b (clon... -
APC/Fire™ 750 anti-mouse Perforin
C57BL/6 splenocytes were stained with anti-mouse CD49b APC. ... -
Pacific Blue™ anti-mouse Perforin
C57 splenocytes were stained with anti-mouse CD49b FITC, fix... -
Alexa Fluor® 647 anti-mouse Perforin
C57 splenocytes were stained with anti-mouse CD49b PE, fixed... -
Brilliant Violet 421™ anti-mouse Perforin
C57BL/6 splenocytes were stained with anti-mouse CD49b APC. ...
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