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Brilliant Violet 421™ anti-mouse/rat XCR1 Antibody

Pricing & Availability
Clone
ZET (See other available formats)
Regulatory Status
RUO
Other Names
GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
ZET_BV421_XCR1_Antibody_FC_1_122214
Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c PE, CD8α APC, and XCR1 (clone ZET) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_BV421_XCR1_Antibody_FC_1_122214
    Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c PE, CD8α APC, and XCR1 (clone ZET) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_BV421_XCR1_Antibody_FC_2_122214
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
148216 50 µg £200
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Description

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α+ conventional dendritic cells capable of antigen cross-presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.

Application References

(PubMed link indicates BioLegend citation)
  1. Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
  2. Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations
  1. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  2. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
  3. Liu L, et al. 2022. Nat Commun. 13:6740. PubMed
  4. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  5. Liu J, et al. 2019. Immunity. 50:600. PubMed
  6. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  7. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  8. Xu H, et al. 2023. Elife. 12:. PubMed
  9. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  10. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  11. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  12. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  13. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
  14. Giampazolias E, et al. 2021. Cell. . PubMed
  15. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  16. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  17. Huang X, et al. 2021. Immunity. . PubMed
RRID
AB_2565230 (BioLegend Cat. No. 148216)

Antigen Details

Structure
Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution

Subset of CD8α+ conventional dendritic cells.

Function
Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor
XCL1 and XCL2.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.

Gene ID
23832 View all products for this Gene ID 301086 View all products for this Gene ID
UniProt
View information about XCR1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 01/02/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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