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Brilliant Violet 421™ anti-mouse VISTA (PD-1H) Antibody

Pricing & Availability
Clone
MIH63 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1, B7-H5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MIH63_BV421_VISTA_Antibody_032718
C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 750 and PD-1H (clone MIH63) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • MIH63_BV421_VISTA_Antibody_032718
    C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 750 and PD-1H (clone MIH63) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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150212 50 µg £200
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Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein that is composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and natural killer cells (NK). It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants due to its ability to preferentially suppress CD4+ T cell-mediated immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse VISTA transfected J558 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. van Os BW, et al. 2023. Eur J Immunol. e2350520:53. PubMed
  2. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID
AB_2728192 (BioLegend Cat. No. 150212)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family.
Distribution

Macrophages, dendritic cells, neutrophils, natural killer cells, and naive and activated T cells.

Function
Negative regulator of the immune response.
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp. Med. 208:577.
3. Flies DB, et al. 2014. J. Clin. Invest. 124:1966.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about VISTA on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03/28/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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