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Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Compare all formats See Brilliant Violet 421™ spectral data
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400157 25 tests £113
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400158 100 tests £245
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Application References

(PubMed link indicates BioLegend citation)
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations
  1. Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed
  2. Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed
  3. Iancu O, et al. 2023. Mol Ther Nucleic Acids. 31:105. PubMed
  4. Gravina A, et al. 2023. Nat Biotechnol. 41:717. PubMed
  5. Campbell KA, et al. 2023. Commun Biol. 6:264. PubMed
  6. Lee HS, et al. 2023. Biol Pharm Bull. 46:542. PubMed
  7. Imai H, et al. 2023. iScience. 26:106822. PubMed
  8. Uneda A, et al. 2021. Acta Neuropathol Commun. 9:29. PubMed
  9. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  10. Girard MC, et al. 2021. Sci Rep. 11:5511. PubMed
  11. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  12. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  13. Eremichev R, et al. 2021. Front Cell Dev Biol. 616893:9. PubMed
  14. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  15. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  16. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  17. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
  18. Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
  19. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  20. Nur Patria Y, et al. 2020. Stem Cell Research. 72.79305556. PubMed
  21. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
  22. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  23. Dayang EZ, et al. 2021. Biomed Pharmacother. 133:111073. PubMed
  24. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  25. Grant R, et al. 2021. Methods Protoc. 4:. PubMed
  26. Wang S, et al. 2021. Parasite. 28:13. PubMed
  27. Dayang EZ, et al. 2019. Front Immunol. 10:1169. PubMed
  28. Tamai K, et al. 2022. Cell Rep Methods. 2:100314. PubMed
  29. van Stigt Thans T, et al. 2020. Journal of Virology. 93(20):e00719-19.. PubMed
  30. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  31. Kim Y, et al. 2021. Oncol Lett. 1.022222222. PubMed
  32. Farhat AM, et al. 2021. Cell Reports. 35(4):109044. PubMed
  33. Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed
  34. Sharma VP, et al. 2021. Nat Commun. 12:7300. PubMed
RRID
AB_10897939 (BioLegend Cat. No. 400157)
AB_11150232 (BioLegend Cat. No. 400158)

Antigen Details

Gene ID
NA

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 510™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA,ChIP
APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Biotin Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Cyanine5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
FITC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
PE Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
APC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
APC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
PE Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
FITC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PerCP Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Brilliant Violet 570™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Alexa Fluor® 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 ICC
Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
GoInVivo™ Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA
FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-A0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
Brilliant Violet 750™ Mouse IgG1, κ isotype Ctrl MOPC-21 FC,ICFC
TotalSeq™-B0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Blue™ 550 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC/Fire™ 810 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 640 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 700 Mouse IgG1, κ isotype Ctrl MOPC-21 FC
Spark Violet™ 538 Mouse IgG1, κ isotype Ctrl MOPC-21 FC
Spark YG™ 581 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark YG™ 593 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 423 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 500 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 810 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-Bn0090 Mouse IgG1, κ Isotype Ctrl MOPC-21 SB
Spark Blue™ 574 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Spark YG™ 570 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Fire™ 806 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Blue™ 515 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Fire™ 780 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 744 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark UV™ 387 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Alexa Fluor® 660 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark PLUS UV395™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Spark Red™ 718 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-B1471 Mouse IgG1, κ Isotype Ctrl MOPC-21 ICPG
Go To Top Version: 4    Revision Date: 06/11/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Brilliant Violet 510™ Mouse IgG1, κ Isotype Ctrl

  • Purified Mouse IgG1, κ Isotype Ctrl

    MOPC-21_ChIP_IgG1-k_control_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • APC Mouse IgG1, κ Isotype Ctrl

  • Biotin Mouse IgG1, κ Isotype Ctrl

  • FITC Mouse IgG1, κ Isotype Ctrl

  • PE Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine5 Mouse IgG1, κ Isotype Ctrl

  • APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • FITC Mouse IgG1, κ Isotype Ctrl (ICFC)

  • PE Mouse IgG1, κ Isotype Ctrl (ICFC)

  • APC Mouse IgG1, κ Isotype Ctrl (ICFC)

  • APC Mouse IgG1, κ Isotype Ctrl (FC)

  • PE Mouse IgG1, κ Isotype Ctrl (FC)

  • FITC Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl

  • PerCP Mouse IgG1, κ Isotype Ctrl

    MOPC-21_PerCP_122607
    Human peripheral blood lymphocytes stained with MOPC-21 PerC...

  • PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 570™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 594 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • GoInVivo™ Purified Mouse IgG1, κ Isotype Ctrl

  • FITC Mouse IgG1, κ Isotype Ctrl

  • PE Mouse IgG1, κ Isotype Ctrl

  • APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • APC Mouse IgG1, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-A0090 Mouse IgG1, κ isotype Ctrl

  • Brilliant Violet 750™ Mouse IgG1, κ isotype Ctrl

  • TotalSeq™-B0090 Mouse IgG1, κ isotype Ctrl

  • TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl

  • APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl

  • Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 550 Mouse IgG1, κ Isotype Ctrl

  • APC/Fire™ 810 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 640 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 700 Mouse IgG1, κ isotype Ctrl

  • Spark Violet™ 538 Mouse IgG1, κ isotype Ctrl

  • Spark YG™ 581 Mouse IgG1, κ Isotype Ctrl

  • Spark YG™ 593 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 423 Mouse IgG1, κ Isotype Ctrl

  • GMP PE Mouse IgG1, κ Isotype Ctrl

  • GMP FITC Mouse IgG1, κ Isotype Ctrl

  • GMP APC Mouse IgG1, κ Isotype Ctrl

  • GMP PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • GMP Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • GMP PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • GMP APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • GMP PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 500 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 810 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-Bn0090 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 574 Mouse IgG1, κ Isotype Ctrl

  • Spark YG™ 570 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 806 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 515 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 780 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 744 Mouse IgG1, κ Isotype Ctrl

  • Spark UV™ 387 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 660 Mouse IgG1, κ Isotype Ctrl

  • Spark PLUS UV395™ Mouse IgG1, κ Isotype Ctrl

  • Spark Red™ 718 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-B1471 Mouse IgG1, κ Isotype Ctrl

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