- Clone
- S17011E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD16, CD32
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S17011E_BV510_CD16_32_Antibody_082323 S17011E_BV510_CD16_32_Antibody_082323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S17011E_BV510_CD16_32_Antibody_082323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD16/32 (clone S17011E) Brilliant Violet 510™ (left) or Rat IgG2b, ĸ Brilliant Violet 510™ isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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156625 | 50 µg | £185 |
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Clone S17011E blocks both clone 93 and 2.4G2 also raised against mouse CD16/32
- RRID
-
AB_3083139 (BioLegend Cat. No. 156625)
Antigen Details
- Structure
- Ig superfamily, 40-60 kD
- Distribution
-
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Ligand/Receptor
- IgG
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
- UniProt
- View information about CD16/32 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD16/32 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
TruStain FcX™ PLUS (anti-mouse CD16/32) | S17011E | FC |
APC/Cyanine7 anti-mouse CD16/32 | S17011E | FC |
PE/Cyanine7 anti-mouse CD16/32 | S17011E | FC |
PE anti-mouse CD16/32 | S17011E | FC |
APC anti-mouse CD16/32 | S17011E | FC |
FITC anti-mouse CD16/32 Antibody | S17011E | FC |
PE/Dazzle™ 594 anti-mouse CD16/32 Antibody | S17011E | FC |
PerCP/Cyanine5.5 anti-mouse CD16/32 Antibody | S17011E | FC |
PE/Cyanine5 anti-mouse CD16/32 | S17011E | FC |
Alexa Fluor® 700 anti-mouse CD16/32 | S17011E | FC |
APC/Fire™ 750 anti-mouse CD16/32 | S17011E | FC |
Brilliant Violet 510™ anti-mouse CD16/32 | S17011E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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TruStain FcX™ PLUS (anti-mouse CD16/32)
BALB/c splenocytes were incubated with TruStain FcX™ PLUS (c... -
APC/Cyanine7 anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with CD45R/B220 PE and CD1... -
PE/Cyanine7 anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with CD45R/B220 APC and CD... -
PE anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with CD45R/B220 APC and CD... -
APC anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with CD45R/B220 PE and CD1... -
FITC anti-mouse CD16/32 Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PE/Dazzle™ 594 anti-mouse CD16/32 Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PerCP/Cyanine5.5 anti-mouse CD16/32 Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PE/Cyanine5 anti-mouse CD16/32
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Alexa Fluor® 700 anti-mouse CD16/32
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
APC/Fire™ 750 anti-mouse CD16/32
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Brilliant Violet 510™ anti-mouse CD16/32
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 ...
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