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Brilliant Violet 605™ anti-mouse CD90.2 (Thy-1.2) Antibody

Pricing & Availability
Clone
53-2.1 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-2.1_BV605_052112
C57BL/6 mouse thymocytes were stained with CD90.2 (clone 53-2.1) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
  • 53-2.1_BV605_052112
    C57BL/6 mouse thymocytes were stained with CD90.2 (clone 53-2.1) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
Compare all formats See Brilliant Violet 605™ spectral data
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140317 125 µL £148
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140318 50 µg £187
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
µl size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).

Application References

(PubMed link indicates BioLegend citation)
  1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
  2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
  3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations
  1. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  2. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  3. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  4. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  6. Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
  7. Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed
  8. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  9. Köchl R, et al. 2020. Elife. 9:00. PubMed
  10. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  11. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  12. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  13. de Lucía Finkel P, et al. 2021. Sci Rep. 11:12233. PubMed
  14. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  15. Lima-Junior DS, et al. 2021. Cell. . PubMed
  16. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  17. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  18. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  19. Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed
  20. Aggio-Bruce R, et al. 2020. Mol Neurobiol. . PubMed
  21. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  22. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  23. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  24. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_11203724 (BioLegend Cat. No. 140317)
AB_11203724 (BioLegend Cat. No. 140318)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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