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Brilliant Violet 650™ anti-human CD314 (NKG2D) Antibody

Pricing & Availability
Clone
1D11 (See other available formats)
Regulatory Status
RUO
Other Names
NKG2D
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1D11_Brilliant-Violet-650_CD314_Antibody_030434
Human peripheral blood lymphocytes were stained with anti-human CD8 (clone SK1) FITC and anti-human CD314 (NKG2D) (clone 1D11) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ isotype control (right). Data shown is gated on the lymphocyte population.
  • 1D11_Brilliant-Violet-650_CD314_Antibody_030434
    Human peripheral blood lymphocytes were stained with anti-human CD8 (clone SK1) FITC and anti-human CD314 (NKG2D) (clone 1D11) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ isotype control (right). Data shown is gated on the lymphocyte population.
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320849 25 tests £148
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320850 100 tests £298
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Description

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8+ T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu J, et al. 1999. Science 285:730.
  2. Wu J, et al. 2000. J. Exp. Med. 192:1059.
  3. Groh V, et al. 2001. Nature Immunol. 2:255.
  4. Wu J, et al. 2002. J. Immunol. 169:1236.
  5. Roberts A, et al. 2001. J. Immunol. 167:5527.
  6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
  7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
  8. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  9. Watanbe M, et al. 2014. Int Immunol. PubMed
RRID
AB_3106074 (BioLegend Cat. No. 320849)
AB_3106074 (BioLegend Cat. No. 320850)

Antigen Details

Structure
C-type lectin
Distribution

NK cells, γ/δ T cells, CD8+ T cells

Function
Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor
MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type
NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.

Gene ID
22914 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/05/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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