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Brilliant Violet 650™ anti-mouse CD317 (BST2, PDCA-1) Antibody

Pricing & Availability
Clone
927 (See other available formats)
Regulatory Status
RUO
Other Names
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
927_BV650_CD317_Antibody_FC_1_091213
C57BL/6 splenocytes were stained with CD45R/B220 PE and CD317 (clone 927) Brilliant Violet 650™ (top) or rat IgG2b Brilliant Violet 650™ isotype control (bottom).
  • 927_BV650_CD317_Antibody_FC_1_091213
    C57BL/6 splenocytes were stained with CD45R/B220 PE and CD317 (clone 927) Brilliant Violet 650™ (top) or rat IgG2b Brilliant Violet 650™ isotype control (bottom).
  • 927_BV650_CD317_Antibody_FC_2_091213
Compare all formats See Brilliant Violet 650™ spectral data
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127019 50 µg £220
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Description

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoid dendritic cells (DCs)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
  2. Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
  3. Rajagopal D, et al. 2010. Blood 115:1949. (Depletion)
  4. Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion)
  5. Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
Product Citations
  1. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  2. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  3. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  4. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  5. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2562477 (BioLegend Cat. No. 127019)

Antigen Details

Structure
Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution

Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.

Function
Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Douglas JL. et al. 2009. J Virol. 83(16):7931
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603
3. Neil SJ. et al. 2008. Nature 451:425

Gene ID
69550 View all products for this Gene ID
UniProt
View information about CD317 on UniProt.org
Go To Top Version: 2    Revision Date: 10/21/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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