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Brilliant Violet 711™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
30-F11_BV711_CD45_Antibody_FC_101414
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 711™ (filled histogram) or rat IgG2b, κ Brilliant Violet 711™ isotype control (open histogram).
  • 30-F11_BV711_CD45_Antibody_FC_101414
    C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 711™ (filled histogram) or rat IgG2b, κ Brilliant Violet 711™ isotype control (open histogram).
Compare all formats See Brilliant Violet 711™ spectral data
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103147 50 µg £180
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sefik E, et al. 2022. Nature. 606:585. PubMed
  2. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  3. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  4. Klapproth E, et al. 2022. Nat Commun. 13:7648. PubMed
  5. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  6. Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed
  7. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  8. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  9. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  10. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  11. Li L, et al. 2023. iScience. 26:106095. PubMed
  12. Park JA, et al. 2023. J Immunother Cancer. 11:. PubMed
  13. Liu SY, et al. 2023. STAR Protoc. 4:102240. PubMed
  14. Banka AL, et al. 2023. Nat Commun. 14:2462. PubMed
  15. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  16. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  17. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  18. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  19. Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
  20. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  21. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  22. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  23. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  24. Hole CR, et al. 2019. Nat Commun. 10:2955. PubMed
  25. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  26. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  27. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  28. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  29. Xu S, et al. 2021. Immunity. . PubMed
  30. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  31. Bachofner M, et al. 2017. Dev Cell. 42:616. PubMed
  32. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  33. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  34. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  35. Zhang SL, et al. 2022. Gut Microbes. 14:2046246. PubMed
  36. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  37. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  38. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  39. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  40. Johann K, et al. 2021. Nat Commun. 12:5981. PubMed
  41. Ivan DC, et al. 2021. Front Immunol. 12:666961. PubMed
  42. Silva DA, et al. 2019. Nature. 565:186. PubMed
  43. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  44. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  45. Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed
  46. Tao Z, et al. 2022. Cells. 11:. PubMed
  47. Olivera GC, et al. 2021. Elife. 10:. PubMed
  48. Lin J, et al. 2021. Cancer Immunol Res. 9:1413. PubMed
  49. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  50. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  51. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  52. McGinley AM, et al. 2020. Immunity. 52(2):342-356. PubMed
  53. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
RRID
AB_2564383 (BioLegend Cat. No. 103147)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 1    Revision Date: 10/20/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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