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Brilliant Violet 711™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 711™
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Product Citations
publications
Strepavidin_BV711_Antibody_FC_041714.jpg
Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 711™.
  • Strepavidin_BV711_Antibody_FC_041714.jpg
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 711™.
See Brilliant Violet 711™ spectral data
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405241 100 µL £159
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 711™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 711™ by 405 nm laser light induces a fluorescence maximum emission of 711 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
0.1 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 711™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 711™ conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 711™ is 340 kD and Streptavidin alone is 52 kD.

Product Citations
  1. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  2. Edwards SC, et al. 2023. J Exp Med. 220: . PubMed
  3. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  4. Ramamurthy E, et al. 2023. Front Mol Neurosci. 15:948456. PubMed
  5. Sun P, et al. 2022. iScience. 25:105202. PubMed
  6. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  7. Gao F, et al. 2023. Immunity. 56:864. PubMed
  8. Lütge M, et al. 2023. Nat Immunol. . PubMed
  9. Monslow J, et al. 2020. Am J Pathol. 1118:190. PubMed
  10. Delbridge ARD, et al. 2021. Front Cell Neurosci. 14:592005. PubMed
  11. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  12. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  13. Ferreirinha P, et al. 2022. Development. 149: . PubMed
  14. Tarke A, et al. 2022. Cell. 185:847. PubMed
  15. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  16. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  17. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  18. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  19. Dan JM, et al. 2021. Science. :371. PubMed
  20. Wells DK, et al. 2020. Cell. 183:818. PubMed
  21. Choi HS, et al. 2020. Cell Rep. 30:2989. PubMed
  22. Seydoux E, et al. 2020. Immunity. 53:98. PubMed
Go To Top Version: 3    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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