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Brilliant Violet 785™ anti-human IL-10 Antibody

Pricing & Availability
Clone
JES3-9D7 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
JES3_9D7_BV785_IL10_Antibody_110223
LPS-stimulated human peripheral blood mononuclear cells (in the presence of monensin) were surface stained with anti-human CD14 FITC, fixed, permeabilized, and then stained with anti-human IL-10 (clone JES3-9D7) Brilliant Violet 785™ (left) or rat lgG1, κ Brilliant Violet 785™ isotype control (right). Data shown was gated on monocyte population.
  • JES3_9D7_BV785_IL10_Antibody_110223
    LPS-stimulated human peripheral blood mononuclear cells (in the presence of monensin) were surface stained with anti-human CD14 FITC, fixed, permeabilized, and then stained with anti-human IL-10 (clone JES3-9D7) Brilliant Violet 785™ (left) or rat lgG1, κ Brilliant Violet 785™ isotype control (right). Data shown was gated on monocyte population.
Compare all formats See Brilliant Violet 785™ spectral data
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501433 25 tests £130
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501434 100 tests £267
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Rhesus, Cynomolgus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS - expressed, recombinant human IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA Capture1-5 or ELISPOT Capture6: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include: immunohistochemical staining12.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
  2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
  3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
  4. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
  5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
  6. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
  7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
  8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
  9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
  10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
  11. Mason GM, et al. 2012. PNAS. PubMed
  12. 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)
RRID
AB_3097257 (BioLegend Cat. No. 501433)
AB_3097257 (BioLegend Cat. No. 501434)

Antigen Details

Structure
Acid-labile cytokine, dimer, 35-40 kD (Mammalian)
Bioactivity
Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation
Production inhibited by IL-4, IL-10
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 1    Revision Date: 10/12/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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