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Brilliant Violet 785™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1.4_BV785_Ly-6C_Antibody_FC_060315
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 785™ (filled histogram). Histograms were gated on myeloid cell populations.
  • HK1.4_BV785_Ly-6C_Antibody_FC_060315
    C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 785™ (filled histogram). Histograms were gated on myeloid cell populations.
Compare all formats See Brilliant Violet 785™ spectral data
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128041 50 µg £196
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Grandjean CL, et al. 2021. Sci Adv. 7:. PubMed
  2. Mancini M, et al. 2021. Sci Rep. 11:21171. PubMed
  3. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  4. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  5. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  6. Alexandre YO, et al. 2022. STAR Protoc. 3:101923. PubMed
  7. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  8. du Halgouet A, et al. 2023. Immunity. 56:78. PubMed
  9. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  10. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  11. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  12. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  13. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  14. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  15. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
  16. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  17. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  18. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  19. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  20. Vicencio JM, et al. 2022. Cell Death Dis. 13:274. PubMed
  21. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  22. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  23. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  24. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  25. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  26. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
  27. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  28. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  29. Clark JT, et al. 2021. eLife. 10:00. PubMed
  30. Sun X, et al. 2020. Cell Metabolism. 31(1):189-206.e8.. PubMed
  31. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
  32. Celorrio M, et al. 2022. Acta Neuropathol Commun. 10:10. PubMed
  33. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  34. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  35. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  36. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  37. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  38. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  39. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
RRID
AB_2565852 (BioLegend Cat. No. 128041)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/08/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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