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Cell-Vive™ CD Cell Separation Buffer, GMP

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Other Names
Separation Buffer, Isolation Buffer
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Cell-Vive_CD_Cell_Separation_Buffer_GMP_1_update2_110122
Human CD3+ cells were isolated from Peripheral Blood Mononuclear cells using Cell-Vive™ CD Cell Separation Buffer (Cat. 420512). The cells were isolated using a MojoSort™ magnet (Cat. 480019) as indicated in the protocol for MojoSort™ Human CD3 T cell Isolation Kit (Cat. 480131) (A) or using a common commercially available magnetic separation column kit from another supplier (B). Purity was verified, before and after isolation, by staining with Brilliant Violet 421™ anti-human CD3 (Clone, UCHT1, Cat. 300434) and APC anti-human CD56 (Clone 5.1H11, Cat. 362504).
  • Cell-Vive_CD_Cell_Separation_Buffer_GMP_1_update2_110122
    Human CD3+ cells were isolated from Peripheral Blood Mononuclear cells using Cell-Vive™ CD Cell Separation Buffer (Cat. 420512). The cells were isolated using a MojoSort™ magnet (Cat. 480019) as indicated in the protocol for MojoSort™ Human CD3 T cell Isolation Kit (Cat. 480131) (A) or using a common commercially available magnetic separation column kit from another supplier (B). Purity was verified, before and after isolation, by staining with Brilliant Violet 421™ anti-human CD3 (Clone, UCHT1, Cat. 300434) and APC anti-human CD56 (Clone 5.1H11, Cat. 362504).
  • Cell-Vive_CD_Cell_Separation_Buffer_GMP_2_update2_110122
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420512 250 mL £172
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Description

Cell-Vive™ CD Cell Separation Buffer, GMP is a cell separation buffer with a chemically-defined formulation without animal-derived components or preservatives. This product is intended to be used with our MojoSort™ magnetic cell separation system or equivalent cell separation products. Cell-Vive™ CD Cell Separation Buffer, GMP is filtered through a 0.2 μm membrane and is a 1X formulation ready to use. This GMP product is for research and further cell-based bioprocessing use only

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP cell culture products are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Low endotoxin level (< 1 EU/mL)
  • Mycoplasma and bacterial/fungal growth testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical Data Sheet (pdf)

Product Details

Formulation
Serum-Free, Chemically-Defined, no Sodium Azide
Endotoxin Level
< 1 EU/mL
Storage & Handling
Store between 2°C - 8°C
Application

Cell Separation - Verified
Application Notes

Use of Cell-Vive™ CD Cell Separation Buffer, GMP is recommended for cell separation using MojoSort™ nanobeads or equivalent separation kits. Cell-Vive™ CD Cell Separation Buffer, GMP is a serum-free chemically-defined formulation without animal derived components such as BSA. Cell-Vive™ CD Cell Separation Buffer, GMP is filtered through a 0.2 μm membrane and is a 1X formulation ready to use. Use the buffer under aseptic conditions as needed.

Additional Product Notes

Chemically-Define formulation Cell-Vive™ CD Cell Separation Buffer, GMP can be used for cell separation following MojoSort™ isolation protocol or equivalent isolation kit protocols.

Disclaimer

BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products

Antigen Details

Gene ID
NA
Go To Top Version: 2    Revision Date: 07/19/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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