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Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads

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CV_GMP_MOJO_Streptavidin_Nanobeads_Image1_073024
CD3+ cells were positively selected from a single cell suspension of human peripheral blood mononuclear cells using Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads and GMP Ultra-LEAF™ Serum Free anti-human CD3 (Clone OKT3) Biotin (Cat. No. 317357). Separations were performed in Cell-Vive™ GMP Chemically Defined Cell Separation Buffer (Cat. No. 420512). Cells were stained with anti-human CD3 (clone SK7) FITC (Cat. No. 344804) and anti-human CD14 (clone 63D3) APC (Cat. No. 367118). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Fire750 (Cat. No. 304062) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305) viability dye.
  • CV_GMP_MOJO_Streptavidin_Nanobeads_Image1_073024
    CD3+ cells were positively selected from a single cell suspension of human peripheral blood mononuclear cells using Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads and GMP Ultra-LEAF™ Serum Free anti-human CD3 (Clone OKT3) Biotin (Cat. No. 317357). Separations were performed in Cell-Vive™ GMP Chemically Defined Cell Separation Buffer (Cat. No. 420512). Cells were stained with anti-human CD3 (clone SK7) FITC (Cat. No. 344804) and anti-human CD14 (clone 63D3) APC (Cat. No. 367118). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Fire750 (Cat. No. 304062) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305) viability dye.
  • CV_GMP_MOJO_Streptavidin_Nanobeads_Image2_073024
    CD3+ cells were negatively isolated from a single cell suspension of human peripheral blood mononuclear cells using Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads and MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480022) Biotin-Antibody Cocktail. Separations were performed in Cell-Vive™ GMP Chemically Defined Cell Separation Buffer (Cat. No. 420512). Cells were stained with anti-human CD3 (clone UCHT1) PE (Cat. No. 300408) and anti-human CD14 (clone HCD14) APC (Cat. No. 325608). The analysis was pre-gated with anti-human CD45 (clone HI30) FITC (Cat. No. 304006) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305) viability dye.
  • CV_GMP_MOJO_Streptavidin_Nanobeads_Image3_073024
    CD56+ cells were positively selected from a single cell suspension of human peripheral blood mononuclear cells using Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads and Cell-Vive™ GMP Ultra-LEAF™ Serum Free anti-human CD56 (clone 5.1H11) Biotin (Cat. No. 480054). Separations were performed in Cell-Vive™ GMP Chemically Defined Cell Separation Buffer (Cat. No. 420512). Cells were stained with anti-human CD56 (clone HCD56) APC (Cat. No. 318310) and anti-human CD3 (clone UCHT1) PE (Cat. No. 300408). The analysis was pre-gated with anti-human CD45 (clone HI30) FITC (Cat. No. 304006) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305) viability dye.
  • CV_GMP_MOJO_Streptavidin_Nanobeads_Image4_073024
    NK cells were negatively isolated from a single cell suspension of human peripheral blood mononuclear cells using Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads and MojoSort™ Human NK Cell Isolation Kit (Cat. No. 480054) Biotin-Antibody Cocktail. Separations were performed in Cell-Vive™ GMP Chemically Defined Cell Separation Buffer (Cat. No. 420512). Cells were stained with anti-human CD56 (clone HCD56) APC (Cat. No. 318310) and anti-human CD3 (clone UCHT1) PE (Cat. No. 300408). The analysis was pre-gated with anti-human CD45 (clone HI30) FITC (Cat. No. 304006) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305) viability dye.
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480196 100 µL £271
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480197 1 mL £791
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Description

Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads can be used for positive or negative selection of targeted cells with biotinconjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.

 

Cell-Vive™ GMP MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

 

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads are manufactured and tested in accordance with USP Chapter <1043>, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016.

 

Specifications and processes include:

  • Low endotoxin level (< 5 EU/mL)
  • Manufactured under animal component-free conditions
  • Lot-specific sterility and mycoplasma testing
  • Batch to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical Data Sheet (pdf)

Product Details

Formulation
Aqueous solution containing 0.3% recombinant HSA
Endotoxin Level
< 5 EU/mL
Preparation
Animal component-free, no preservatives
Storage & Handling
4°C (2°C-8°C)
Application

Cell Sep-Neg - Quality tested

Cell Sep-Pos - Verified in house
Recommended Usage

The concentration of Streptavidin Nanobeads to be used should be optimized by end users.

Application Notes

The Streptavidin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.

Application References

(PubMed link indicates BioLegend citation)
  1. Guo H, et al. 2016. PLoS One. 11(3):e0150809. (PubMed)
Disclaimer

BioLegend Cell-Vive™ GMP MojoSort™ Streptavidin Nanobeads are for research use only. Suitable for ex vivo cell processing. Not for use in diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 07/31/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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