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Cell-Vive™ GMP Ultra-LEAF™ Purified anti-HU CD3 RecAb SF

Pricing & Availability
Clone
OKT3.Rec (See other available formats)
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OKT3-Rec_CV_GMP_ULEAF_CD3_RecAb_SF_image1_102324
Human peripheral blood lymphocytes were stained with Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD3 RecAb SF at 0.25 µg per test (Clone OKT3.Rec, cat#: 317368, filled histogram) or an isotype control (Clone MOPC-173, cat#: 400201, open histogram). Both primary stains were followed by an anti-mouse IgG FITC secondary stain (Clone Poly4060, cat#: 406001).
  • OKT3-Rec_CV_GMP_ULEAF_CD3_RecAb_SF_image1_102324
    Human peripheral blood lymphocytes were stained with Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD3 RecAb SF at 0.25 µg per test (Clone OKT3.Rec, cat#: 317368, filled histogram) or an isotype control (Clone MOPC-173, cat#: 400201, open histogram). Both primary stains were followed by an anti-mouse IgG FITC secondary stain (Clone Poly4060, cat#: 406001).
  • OKT3-Rec_CV_GMP_ULEAF_CD3_RecAb_SF_image2_102324
    PBMC-derived T cells were activated in the presence of Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD3 RecAb SF antibody (clone OKT3.Rec, cat#: 317368, black bar) alone or combined with Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD28 RecAb SF antibody (clone S20013F.Rec, cat#: 377614, purple bar). These conditions were compared to both: OKT3.Rec combined with GMP Ultra-LEAF Purified anti-human CD28 SF antibody (clone S20013F, cat#: 377605, pink bar) and S20013F.Rec combined with GMP Ultra-LEAF Purified anti-human CD3 SF Antibody (clone: OKT3, cat#: 317353, teal bar). T cells were activated by plating 1x106 cells on a 12-well plate coated with 1 µg/mL of each antibody in PBS. Cells were cultured for a total of 10 days and fold expansion compared to D0 was calculated at D10. Statistical significance was observed for each antibody pair compared to Cell-Vive™ GMP Ultra-LEAF™ Purified anti-human CD3 RecAb SF antibody alone. Unpaired t-tests confirmed no significant difference in fold expansion between antibody pairs.
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317367 100 µg £225
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317368 1 mg £1152
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Ultra-Low endotoxin level (<0.01 EU/µg)
  • Manufactured under serum-free conditions
  • Bulks tested negative for mycoplasma and bacterial/fungal growth
  • Serum-free hybridomas are negative for the presence of murine virus
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
GMP Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant Serum Free antibody purified by affinity chromatography
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Activ - Verified in house

Costim - Reported in the literature
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

OKT3.Rec antibody can be used at 1 µg/mL of antibody in PBS alone or in combination with 1 µg/mL of anti-human CD28 antibody for T cell activation.

Application Notes

The OKT3.Rec recombinant antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

 

Clone OKT3.Rec can block the binding of clones SK7 and UCHT1. The OKT3.Rec antibody is able to induce T cell activation.

 

This GMP Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.1 µm filtered) is recommended for highly sensitive assays.

Disclaimer

BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur J Immunol. 11:329.
  3. Lanier L, et al. 1986. J Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 1    Revision Date: 11/05/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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