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- Regulatory Status
- RUO
- Other Names
- Compensation, Comp Beads
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Kit content image for the Compensation Beads -
Compensation Beads were stained with 23 different FITC-conjugated isotypes of antibodies of mouse, rat, rabbit, donkey, hamster and human origin. Beads were stained with 0.05 µg of antibody per test.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 424601 | 25 tests | £70 | ||||
| 424602 | 100 tests | £161 | ||||
Compensation Beads can bind fluorescently tagged mouse, rat, rabbit, donkey, hamster and human antibodies. Each kit contains 1 dropper bottle of negative beads and 1 dropper bottle of positive beads. The positive beads will bind any mouse, rat, rabbit, donkey, hamster or human antibody. Antibodies from other host species have not been verified to bind to the positive beads. The negative beads will not bind any antibody. Compensation Beads can be used as compensation controls for multicolor flow cytometry assays.
Compensation Beads can be used for dyes excited by blue (488 nm), green (532 nm), yellow/green (561 nm), violet (405 nm), ultraviolet (355 nm), and red (633-635 nm) lasers.
Product Details
- Formulation
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Each kit will contain sets of two dropper bottles as listed below:
1. Negative beads
2. Positive beads
- Preparation
- Compensation Beads are supplied ready to use, 1 drop (~25-30 µL) from each bottle is sufficient for one test.
- Storage & Handling
- Store the kit at 2°C - 8°C upon receipt.
- Application
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FC - Quality tested
- Recommended Usage
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1 drop of positive beads plus one drop of negative beads
- Application Notes
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Compensation Beads can bind mouse, rat, rabbit, donkey, hamster and human monoclonal or polyclonal antibodies. Antibodies from other host species have not been verified to bind to the positive beads and may not be compatible for use with this product. Product is a kit of one positive beads bottle and one negative beads bottle. Compensation Beads can be used as compensation controls for multicolor flow cytometry assays or in any other assay that require antibody binding beads.
Protocol for using Compensation Beads:- Vortex beads vigorously for 1-2 minutes prior to using to disrupt aggregates that may have formed during storage.
- Prepare tubes with appropriate antibodies (single color).
- Add one drop of beads from each bottle (positive and negative). Mix thoroughly. Ensure that any stray liquid on the side of the tube is mixed in with the antibody.
- Incubate at room temperature for 15-20 minutes, protected from exposure to light.
- Wash beads with Cell Staining Buffer (Cat. No. 420201) or equivalent.
- Centrifuge at 350Xg for 5 minutes and re-suspend in Cell Staining Buffer or equivalent. Beads are ready to acquire.
Antigen Details
- Gene ID
- NA
Related FAQs
- I observed multiple positive peaks when running Compensation Beads. What could be causing this?
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Multiple positive peaks can occur from insufficient vortexing or mixing of compensation beads. Be sure to vigorously vortex beads for at least one minute before dispensing drops. Longer vortexing helps to disrupt aggregates that can form during storage. Also, ensure that any stray droplets sticking to the side of the tube are thoroughly mixed in with your sample before incubation. If you have already prepared your single-color controls and do not wish to remake them, try setting your positive gate on the major peak, excluding minor stained populations.
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