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Cyto-Last™ Buffer

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Regulatory Status
RUO
Other Names
CytoLast Buffer
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Product Citations
publications
CytoLastBuffer_MQ1-17H12_PE1_070312
PMA+ionomycin-stimulated human peripheral blood lymphocytes (for 6 hours in the presence of monensin) were surface stained with CD3 APC and fixed. Cells were then permeabilized and intracellularly stained with human IL-2 (clone MQ1-17H12) PE at day 0 (top), or at day 14 (bottom) after storage in BioLegend's Cyto-Last™ Buffer.
  • CytoLastBuffer_MQ1-17H12_PE1_070312
    PMA+ionomycin-stimulated human peripheral blood lymphocytes (for 6 hours in the presence of monensin) were surface stained with CD3 APC and fixed. Cells were then permeabilized and intracellularly stained with human IL-2 (clone MQ1-17H12) PE at day 0 (top), or at day 14 (bottom) after storage in BioLegend's Cyto-Last™ Buffer.
  • CytoLastBuffer_MQ1-17H12_PE2_070312
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422501 100 mL £53
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Description

Cyto-Last™ Buffer is specially formulated for the storage of cytokine producing cells. When used, staining of intracellular and/or extracellular targets for flow cytometric analysis can be delayed for up to two weeks. Cells should be stored at 4°C during this time. This unique buffer ensures cells maintain a background staining signal equal to that of freshly prepared cells, while also retaining high specific immunofluorescent staining against target antigens.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store Cyto-Last™ Buffer between 2°C and 8°C.
Application

ICFC - Quality tested
Application Notes

Staining Procedure:
1. Prepare target cells of interest and perform surface staining as described in BioLegend's Cell Surface Immunofluorescence Staining Protocol. (Note: staining with tandem-dye-conjugated antibodies (e.g., PE/Cyanine7, APC/Cyanine7, etc.) is not recommended as color compensation shifts may occur with long-term storage.)
2. Fix the cells with 0.5 mL/tube BioLegend's Fixation Buffer (Cat. No. 420801) at room temperature, in the dark, for 20 minutes.
3. Centrifuge at 350 x g for 5 minutes; discard supernatant.
4. Resuspend the cells in 0.5-1 mL/tube Cyto-Last™ Buffer, mix, cap the tubes, and then store at 4°C in the dark (Note: Cyto-Last™ Buffer can also be used for preserving cells in bulk at a cell concentration of 0.5-2.0 x 106 cells/mL.).
5. Take out the tubes at desired time points. Remove Cyto-Last™ Buffer by centrifugation, permeabilize the cells with BioLegend's Permeabilization Wash Buffer (Cat. No. 421002), and perform intracellular cytokine staining.

Product Citations
  1. Parveen S, et al. 2023. Nat Commun. 7427:14. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 2    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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