Pricing & Availability
Technical Data Sheet (pdf)
- Regulatory Status
- RUO
- Other Names
- Chromogen Substrate Solution
- Previously
-
Covance Catalog# SIG-31048
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
926801 | 1 kit | £73 |
Kit Contents
- Kit Contents
-
• DAB Chromogen, 3mL
• DAB Substrate Buffer w/Stabilizer, 50mL
Product Details
- Formulation
- Chromogen Substrate Buffer.
- Storage & Handling
- Store between 2-8°C. DAB preparations should be stored in a manner which minimizes exposure to light.
- Application
-
IHC
- Recommended Usage
-
Protocol:
1. Add the DAB chromogen to the DAB substrate buffer as described in the table below
Buffer DAB Chromogen
5 mL + 0.2 mL
25 mL + 1.0 mL
50 mL + 2.0 mL
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background - Application Notes
-
BioLegend’s DAB 2-Component w/Stabilizer consists of a concentrated (25x) DAB chromogen and a Stable buffer. The DAB solution reacts to give a brown staining with minimal background noise.
Antigen Details
- Gene ID
- NA
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