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- Clone
- P1A12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glutathione-S-transferase
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
10 µg of recombinant GST protein tagged with HA-tag was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP GST (Clone P1A12). Proteins were visualized using chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 640804 | 25 µL | £70 | ||||
| 640803 | 100 µL | £233 | ||||
GST catalyzes the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles, resulting in the detoxification of these compounds.Human GST molecules are classified into four families: alpha, mu, pi, and theta.GST is commonly used as a tag to create fusion proteins in order to facilitate purification and detection of these proteins of interest.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full length GST
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. -
Application References
(PubMed link indicates BioLegend citation) -
- Nicol C, et al. 2011. Biochem Biophys Res Commun. 405:417. PubMed
- RRID
-
AB_2572007 (BioLegend Cat. No. 640804)
AB_2572007 (BioLegend Cat. No. 640803)
Antigen Details
- Structure
- MW, 22-29 kD
- Function
- GST catalyzes the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles, resulting in the detoxification of these compounds.
- Biology Area
- Cell Biology
- Antigen References
-
1. Dejong JL, et al. 1988. Nucleic Acids Res. 16:8541.
2. Alin P, et al. 1985. FEBS Lett. 182:319 - Gene ID
- 56615 View all products for this Gene ID
- UniProt
- View information about GST on UniProt.org
Related FAQs
Other Formats
View All GST Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-GST | P1A12 | WB,Purification |
| Direct-Blot™ HRP anti-GST | P1A12 | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GST
Western blot analysis of recombinant GST protein from E. ... -
Direct-Blot™ HRP anti-GST
10 µg of recombinant GST protein tagged with HA-tag was reso...
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