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Direct-Blot™ HRP anti-TCF1 (TCF7) Antibody

Pricing & Availability
Clone
7F11A10 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell-specific transcription factor 1, transcription factor 7, TCF7
Isotype
Mouse IgG1, κ
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Product Citations
publications
7F11A10_DB_HRP_TCF1_Antibody_1_060118
Western blot analysis of 15µg cell lysates from Jurkat (highly expressed) and HepG2 (lowly expressed) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.025, 0.1 and 0.5 µg/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-TCF-1 antibody, clone 7F11A10. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 7F11A10_DB_HRP_TCF1_Antibody_1_060118
    Western blot analysis of 15µg cell lysates from Jurkat (highly expressed) and HepG2 (lowly expressed) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.025, 0.1 and 0.5 µg/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-TCF-1 antibody, clone 7F11A10. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 7F11A10_DB_HRP_TCF1_Antibody_2_060118
    Western blot analysis of 15µg cell lysates from Jurkat (highly expressed) and HepG2 (lowly expressed) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.5, 1.5 and 2 µg/ml Direct-Blot™ HRP, or 2 µg/ml purified anti-TCF-1 antibody, clone 7F11A10. Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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655209 25 µL £70
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655210 100 µL £233
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Description

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial TCF1 recombinant protein (116-334 aa)
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:250-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734521 (BioLegend Cat. No. 655209)
AB_2734521 (BioLegend Cat. No. 655210)

Antigen Details

Structure
384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution

Nucleus

Function
TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction
TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
TCRs, Nuclear Markers
Antigen References

1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207.
2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060.
3. Wang R, et al. 2011. J. Immunol. 187:5964.
4. Weber BN, et al. 2011. Nature 476:63.
5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777.
6. Staal FJ, et al. 1999. Int. Immunol. 11:317.

Gene ID
6932 View all products for this Gene ID
UniProt
View information about TCF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/04/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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