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Dry Human IP Backbone Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Dry Reagents, Dry Cocktails
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Product Citations
publications
1_Human_IP_Backbone_Kit_1_062723
Human peripheral blood lymphocytes were stained with Dry Human IP Backbone Cocktail and gated on CD45+ lymphocytes. Data shown for immune cell subsets include CD3+, CD19+, CD16/CD56+.
  • 1_Human_IP_Backbone_Kit_1_062723
    Human peripheral blood lymphocytes were stained with Dry Human IP Backbone Cocktail and gated on CD45+ lymphocytes. Data shown for immune cell subsets include CD3+, CD19+, CD16/CD56+.
  • Human_IP_Backbone_Kit_2_062723
    Human peripheral blood monocytes were stained with Dry Human IP Backbone kit and gated on CD45+ monocytes. Data shown for immune cell subsets include CD14+ and CD11c+.
  • Human_IP_Backbone_Kit_3_062723
    Human peripheral granulocytes were stained with Dry Human IP Backbone Cocktail and gated on CD45+ granulocytes. Data shown for immune cell subsets include CD15+ and CD11c+.
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320303 5 tests £136
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Description

Dry Human IP (Immunophenotyping) Backbone Kit is a unit dose cocktail for characterizing immune cell subsets. PE and APC channel are left open by design to allow end users to add antibodies against their additional target of interest. The Dry Human IP Backbone Kit may be a used a stand-alone or as a backbone to which additional antibodies may be added for specific applications. The kit contains 5 tests of Human IP Backbone Cocktail (1 test / tube) and 1 set of compensation controls with lot-matched antibodies.

The Dry Human IP Backbone Cocktail contains:

Spark Violet™ 538 anti-human CD45 (Clone HI30)
FITC anti-human CD15 (Clone W6D3)
PerCP/Cyanine5.5 anti-human CD14 (Clone QA18A22)
Alexa Fluor® 700 anti-human CD3 (Clone SK7)
PE/Cyanine7 anti-human CD19 (Clone SJ25C1)
APC/Fire™ 750 anti-human CD11c (Clone Bu15)
PE/Dazzle™ 594 anti-human CD56 (Clone 5.1H11)
PE/Dazzle™ 594 anti-human CD16 (Clone B73.1)

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Formulation
Each kit contains the following:

5 Tubes of Dry Human IP Backbone Cocktail (1 test/tube)
1 Compensation Control Tube for Spark Violet™ 538 (1 test/tube)
1 Compensation Control Tube for Alexa Fluor® 700 (1 test/tube)
1 Compensation Control Tube for PerCP/Cyanine5.5 (1 test/tube)
1 Compensation Control Tube for APC/Fire™ 750 (1 test/tube)
1 Compensation Control Tube for PE/Cyanine7 (1 test/tube)
1 Compensation Control Tube for PE/Dazzle™ 594 (1 test/tube)
1 Compensation Control Tube for FITC (1 test/tube)

Dry cocktail is prepared from PBS containing stabilizers. Each tube can be reconstituted with 100 µL of whole blood/cell suspension or with 50 µL Cell Staining Buffer plus 50 µL of cell suspension.
Preparation
This kit is a combination of conjugated antibodies at optimal concentrations for flow cytometry application.
Storage & Handling
The product can be stored at ambient temperature upon receipt.

Do not open the pouch until ready to use. Once opened, product can be stored at ambient temperature for up to a month in the mylar pouch.

If product is reconstituted with buffer, it can be stored in the fridge (2 - 8°C) until it is ready to use (up to 1 week).
Application

FC - Quality tested
Recommended Usage

Please refer to Application Notes

Application Notes

Dry Human IP (Immunophenotyping) Backbone Kit is a dried down cocktail kit to characterize immune cell subsets.

Components:
1 pouch containing 5 tests of Dry Human IP Backbone Cocktail (1 test/tube)
1 set of Compensation Control tubes with lot-matched antibodies

Required Materials Not Included:
Cell Stain Buffer for Wash Steps (Cat. No. 420201 or similar)
1x RBC Lysis Buffer diluted from 10x RBC Lysis Buffer (Cat. No. 420302 or similar)
1% diluted Fixation Buffer from 4% if needed (Cat. No. 420801 or similar)

Assay Protocol:

  1. Open the Dry Human IP Backbone Kit pouches and the respective compensation control pouches and set up the dried down cocktail tubes to start the experiment
  2. Add 100 µL of human peripheral blood or similar cell suspension of interest into each of the dried down cocktail tubes and respective compensation controls and vortex thoroughly
  3. Incubate the samples at room temperature for 15 minutes
  4. If the cell suspension used does not need lysing, proceed to step 7. If performing Lyse wash whole blood assay, add 2 mL of 1x of RBC Lysis Buffer (Cat. No. 420302 or similar) to each tube and vortex thoroughly
  5. Incubate the samples at room temperature for 15 minutes
  6. Centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet
  7. Add 2 mL Cell Stain Buffer (Cat. No. 420201 or similar) and centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet
  8. Repeat step 7
  9. Add 300 – 500 µL Cell Stain Buffer or 1% diluted Fixation Buffer (Cat. No. 420801 or similar) and acquire the samples using a flow cytometer
RRID
AB_3097207 (BioLegend Cat. No. 320303)

Antigen Details

Distribution

T Cells, B cells, Granulocytes, Monocytes, NK Cells & Dendritic Cells

Molecular Family
CD Molecules
Gene ID
NA
Go To Top Version: 1    Revision Date: 06/27/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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