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DyLight™ 594 Goat anti-hamster (Armenian) IgG Antibody

Pricing & Availability
Clone
Poly4055 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly4055_Dylight594_hamster__Antibody_102820
Mouse splenocytes were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 10 µg/mL anti-mouse CD3ε Antibody (145-2C11) Antibody and followed by DyLight™ 594 goat anti-hamster IgG (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • Poly4055_Dylight594_hamster__Antibody_102820
    Mouse splenocytes were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 10 µg/mL anti-mouse CD3ε Antibody (145-2C11) Antibody and followed by DyLight™ 594 goat anti-hamster IgG (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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405504 100 µg £73
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Description

This polyclonal antibody reacts with the heavy chains of IgG and the light chains common to most immunoglobulins from Armenian hamster. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with mouse, rat, human, bovine, and rabbit immunoglobulins, but may cross-react with Armenian hamster IgM and Syrian hamster immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Armenian Hamster
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with DyLight™ 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Verified
Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight™ 594  has a maximum absorption of 592nm and a maximum emission of 617 nm (Alexa Fluor® 594 or Texas Red equivalent). Prior to using DyLight™594 conjugates, please verify your instrument's capability of exciting and detecting the fluorochrome.

Application Notes

This conjugated goat anti-hamster IgG antibody is useful for immunofluorescent staining with flow cytometric analysis or microscopy.

Product Citations
  1. Boroujerdi A, et al. 2013. Exp Neurol. 250:43. PubMed
  2. Jeon H, et al. 2021. Neurotherapeutics. 18:2692. PubMed
  3. Meyer N, et al. 2017. Sci Rep. 10.1038/srep45106. PubMed
  4. Tanida S, et al. 2013. J Biol Chem. 288:31842. PubMed
  5. Willis S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01605-1. PubMed
  6. Baba T, et al. 2020. eLife. 9:e54257.. PubMed
RRID
AB_1575119 (BioLegend Cat. No. 405504)

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Go To Top Version: 5    Revision Date: 10/28/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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