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- Regulatory Status
- RUO
- Other Names
- Obese Protein, Obesity Factor, LEPD, OB, OBS
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 444304 | 5 plates | £257 | ||||
Human Leptin is a 167 amino acid protein and approximately 16 kD in mass. Leptin is encoded by the obese (ob) gene and the gene locates on chromosome 7. Leptin is an adipocyte-derived hormone and mainly secreted by adipocyte. Small amounts of Leptin are expressed by the cells in the epithelium of the stomach and in the placenta. Leptin’s effects on body weight are mediated through impacts on hypothalamic center that control feeding behavior, hunger, body temperature, and energy expenditure. Leptin importantly regulates food intake, body weight, metabolism, energy expenditure, reproductive function, and adiposity through hypothalamic Leptin receptor. Leptin is also the member of long-chain helical cytokine family.
Product DetailsKit Contents
- Kit Contents
-
- Human Leptin Capture Antibody (200X)
- Human Leptin Detection Antibody (200X)
- Human Leptin Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer B (5X)
- Assay Diluent B (5X)
- Blocking Buffer A (2X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 10.5 pg/mL
- Standard Range
- 15.6 - 1000 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Endothelial cells, Fibroblasts
- Biology Area
- Angiogenesis, Cell Biology, Signal Transduction
- Molecular Family
- Enzymes and Regulators
- Gene ID
- NA
- UniProt
- View information about Leptin on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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