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ELISA MAX™ Deluxe Set Human Neurogranin

Pricing & Availability
Regulatory Status
RUO
Other Names
Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng, NRGN
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Product Citations
publications
Elisa-Max_Human-Neurogranin_ELISA_100924
  • Elisa-Max_Human-Neurogranin_ELISA_100924
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430004 5 plates £250
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Description

Neurogranin (NRGN) is the main post-synaptic protein kinase substrate that binds to calmodulin (CaM) in the absence of calcium. NRGN’s size (78 amino acids, 7.6 kD) allows it to pass through a damaged blood–brain barrier, making it a biomarker in chronic neurodegenerative diseases and potentially for acute brain injury. Measurement of serum NRGN levels could support the early diagnosis of central nervous infections.

Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease. NRGN function has been implicated in learning and memory. It is suggested that the NRGN is a direct target for thyroid hormone in the human brain and the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development, as well as in adult subjects.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Neurogranin Capture Antibody (200X) 
  • Human Neurogranin Detection Antibody (200X) 
  • Human Neurogranin Lyophilized Standard 
  • Avidin-HRP (1000X) 
  • Substrate Solution F 
  • Coating Buffer A (5X) 
  • Blocking Buffer A (2X) 
  • Assay Diluent B (5X) 
  • Assay Diluent D

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
3.46 ng/mL
Standard Range
6.25 - 400 ng/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20 
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4 
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered 
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle or automated microplate washer 
  • Log-Log graph paper or software for data analysis 
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
NA
UniProt
View information about Neurogranin on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 09/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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