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- Regulatory Status
- RUO
- Other Names
- Human Phospho-EGFR (Y 1173), Human EGFR (pTyr1173), Human EGFR (pY1173)
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- Product Citations
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ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr1173) Standard Curve -
The quantification of Human phospho-EGFR (Tyr1173) is demonstrated by treating A431 cells with or without the EGFR kinase inhibitor PD153035. A431 cells were incubated with or without recombinant human EGF (Cat. No. 585506) at 100 ng/mL after being pre-treated with or without the EGFR-kinase inhibitor PD153035 at either 20 nM or 100 nM. Cells were collected and then lysed with ice-cold lysis buffer. BCA assay was performed to measure total protein concentration. All lysate samples were diluted down to 10 µg/mL total protein in 1X ADB before being analyzed with the ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr1173) assay. Then, the measured phospho-EGFR (Tyr1173) was normalized to the total protein concentration.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 449604 | 5 plates | £425 | ||||
EGFR (Epidermal Growth Factor Receptor) is a member of the ErbB/HER family of cell surface receptors which comprises of four closely related tyrosine kinase receptors: EGFR/HER1, HER2, HER3, and HER4. Binding of a ligand to the extracellular domain of EGFR results in receptor dimerization and autophosphorylation on tyrosine residues with the major phosphorylation sites being tyrosine 1068 (Tyr1068), Tyr1148, and Tyr1173. Phosphorylation of Tyr1173 leads to interaction with Shc and phospholipase Cγ (PLCγ), which are involved in the activation of MAPK signaling pathway. EGFR activation triggers intracellular signaling that results in increased proliferation and a number of processes related to cell survival. ErbB receptors are overexpressed or mutated in many cancers. It was reported that deficient signaling of the EGFR can result in degenerative diseases such as Alzheimer’s disease.
BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant human Phospho-EGFR (Tyr1173). The set is designed for cost-effective and accurate quantification of human Phospho-EGFR (Tyr1173) in cell and tissue lysate. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.
Kit Contents
- Kit Contents
-
- Human Phospho-EGFR (Tyr1173) ELISA MAX™ Capture Antibodies (200X)
- Human Phospho-EGFR (Tyr1173) ELISA MAX™ Detection Antibodies (200X)
- Human Phospho-EGFR (Tyr1173) Lyophilized Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Assay Diluent B (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.17 ± 0.07 ng/mL
- Standard Range
- 0.47 - 30 ng/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Endothelial cells, Epithelial cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Signal Transduction, Stem Cells
- Molecular Family
- Growth Factors, Phospho-Proteins
- Gene ID
- P00533 View all products for this Gene ID
- UniProt
- View information about Phospho-EGFR Tyr1173 on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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