- Regulatory Status
- RUO
- Other Names
- Human Phospho EGFR (Y 845), Human EGFR (pTyr845), Human EGFR (pY845)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
449804 | 5 plates | £442 |
EGFR (Epidermal Growth Factor Receptor) is a member of the ErbB family of cell surface receptors. It is an oncoprotein which has been found to have increased expression and activity in multiple cancer types. However, low activity of EGFR can result in degenerative diseases such as Alzheimer’s disease. EGFR signaling pathway starts when it binds to the ligand EGF, resulting in receptor dimerization, transphosphorylation of the C-terminal tails, and propagation of the signal downstream. Each phosphorylation site can serve as a docking site for different proteins of different signaling pathways. Phosphorylation of tyrosine 845 is associated with regulation of receptor function and tumor progression via c-Src in a Zn2+-dependent manner.
BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant human Phospho-EGFR (Tyr845). The set is designed for cost-effective and accurate quantification of human Phospho-EGFR (Tyr845) in cell and tissue lysates. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.
Kit Contents
- Kit Contents
-
- Human Phospho-EGFR (Tyr845) ELISA MAX™ Capture Antibody (200X)
- Human Phospho-EGFR (Tyr845) ELISA MAX™ Detection Antibody (200X)
- Human Phospho-EGFR (Tyr845) Lyophilized Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Assay Diluent B (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.064 ± 0.036 ng/mL
- Standard Range
- 0.16 - 10 ng/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Epithelial cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Gene ID
- NA
- UniProt
- View information about EGFR Tyr845 on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
Follow Us