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Epitope Tag Small Motif Antibody Sampler Kit

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C_SmallMotifSampler_HA11_WB_072717
15µg each of total protein from CHO cells (lane 3), and CHO transfected with HA tagged protein (lane 4) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-HA.11 Epitope Tag (clone 16B12) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) Antibody was used as a loading control. Lane M: MW ladder. Lane M*: long exposure MW ladder.
  • C_SmallMotifSampler_HA11_WB_072717
    15µg each of total protein from CHO cells (lane 3), and CHO transfected with HA tagged protein (lane 4) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-HA.11 Epitope Tag (clone 16B12) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) Antibody was used as a loading control. Lane M: MW ladder. Lane M*: long exposure MW ladder.
  • SmallMotifSampler_WB_012522
    50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with either 1 µg/mL of Purified anti-DYKDDDDK Tag (clone L5) Antibody, 0.5 ug/ml of anti-C-Myc (clone 9E10) antibody, 0.2 µg/mL of Purified anti-His Tag (clone J099B12) or anti-V4-tag (clone 7/4) antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder. Lane M*: long exposure MW ladder.
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Description

The Epitope Tag (Small Motif) Antibody Sampler Kit provides a convenient resource to detect small Epitope Tag fused protein by Western Blot. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. Anti-His tag antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. For additional antigen details, please refer to individual product datasheets.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD)
Anti-DYKDDDDK L5 25 µg DYKDDDDK tag epitope Rat IgG2a, κ N/A
Anti-c-Myc 9E10 25 µg EQKLISEEDL tag epitope Mouse IgG1 N/A
Anti-HA.11 16B12 25 µg YPYDVPDYA tag epitope Mouse IgG1, κ N/A
Anti-V5 Tag 7/4 25 µg GKPIPNPLLGLDST tag epitope Mouse IgG N/A
Anti-His Tag J099B12 25 µg 6x His tag epitope Mouse IgG1, κ N/A

* For detailed information about each specificity, please follow the provided links. 

Product Details

Formulation
Please refer to individual product datasheets for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Concentration
All antibodies in the kit are at 0.5mg/ml
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-DYKDDDDK: 0.1-1 µg/ml
Anti-Myc: 0.1-0.5 µg/ml
Anti-HA.11: 0.1-1 µg/ml
Anti-V5 tag: 0.1-0.5 µg/ml
Anti-His tag: 0.1-0.2 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed (Anti-DYKDDDDK)
  2. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed (Anti-DYKDDDDK)
  3. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed (Anti-DYKDDDDK)
  4. Das A, et al. 2016. J. Biol. Chem.. 291: 6096 - 6110. PubMed (Anti-DYKDDDDK)
  5. Kondo, S., et al. 2011. J. Virol. 85:11255. PubMed (Anti-Myc)
  6. Bourgeois-Daigneault MC, et al. 2012. J Immunol. 188:4959. PubMed (Anti-Myc)
  7. Robu M, et al. 2013. PNAS. 110:1658. PubMed (Anti-Myc)
  8. Pecot MY, Malhotra V. 2004. Cell. 116:99. (Anti-HA.11)
  9. Görtz D, et al. 2015. Scientific Reports. 5: 14685. PubMed (Anti-HA.11)
  10. Thoms M, et al. 2016. Nucleic Acids Res. 44: 926 - 939. PubMed (Anti-HA.11)
  11. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. (Anti-His tag)
  12. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429. (Anti-His tag)
  13. Keshwani M, et al. 2015. Biochem. J.. 472: 329 - 338. PubMed (Anti-His tag)

Antigen Details

Biology Area
Cell Biology
Gene ID
4609 View all products for this Gene ID

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Go To Top Version: 1    Revision Date: 07/26/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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