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FITC Annexin V

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Regulatory Status
RUO
Other Names
Annexin A5
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AnnexinV_FITC_092908.jpg
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • AnnexinV_FITC_092908.jpg
    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)
  • B_AnnexinV_FITC_092908.jpg
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640905 25 tests £57
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640906 100 tests £133
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640945 300 tests £278
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Description

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our Helix NP™ Blue (Cat. No. 425305), Helix NP™ Green (Cat.  No. 425303) or Helix NP™ NIR (Cat. No. 425301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Other Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The purified protein was conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired.
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References

(PubMed link indicates BioLegend citation)
  1. Koopman G, et al. 1994. Blood 84:1415.
  2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
  4. Sekine C, et al. 2009. Int Immunol. PubMed
  5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
  6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
  7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  8. Speth C,et al. 2013. J Infect Dis. 207:823. PubMed
  9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
  11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed
  13. Sequier S, et al. 2013. PLoS One. 8:70937. PubMed
  14. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  15. Chen YL, et al. 2013. J Exp Med. PubMed
  16. Lin WH, et al. 2014. PLoS One. 9:83160. PubMed
  17. Salge-Bartles U, 2014. Thromb res. 133:244. PubMed
  18. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  19. Intermann J, et al. 2014. Cell Signal. 26:1567. PubMed
  20. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  21. M Parrula MC, et al. 2014. Virus Res. 189:206. PubMed
  22. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  23. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  24. Awojoodu AO, et al. 2014. Blood. 124:1941. PubMed
  25. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  26. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
Product Citations
  1. Derudder E, et al. 2016. Proc Natl Acad Sci U S A. 113: 5065-5070. PubMed
  2. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  3. Yao Z, et al. 2018. J Virol. 92:00:00. PubMed
  4. Uriostegui–Arcos M, et al. 2020. Open Biol. 10:200050. PubMed
  5. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  6. Cao H, et al. 2022. Br J Haematol. 198:155. PubMed
  7. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  8. Cao L, et al. 2022. Cell Rep. 40:111156. PubMed
  9. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  10. Kimmerling RJ, et al. 2022. Commun Biol. 5:1295. PubMed
  11. Chen X, et al. 2022. Front Pharmacol. 13:1028692. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Önel T, et al. 2023. Naunyn Schmiedebergs Arch Pharmacol. :. PubMed
  14. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Li J, et al. 2023. J Biol Chem. 299:102875. PubMed
  16. Martinelli M, et al. 2023. iScience. 26:105860. PubMed
  17. Tay HG, et al. 2023. Mol Ther. 31:825. PubMed
  18. Dean BD, et al. 2023. J Immunother Cancer. 11: . PubMed
  19. Lieschke E, et al. 2022. Cell Death Differ. 29:1004. PubMed
  20. Gallego-Murillo JS, et al. 2022. Biotechnol Bioeng. 119:3096. PubMed
  21. Du X, et al. 2023. Nucleic Acids Res. 51:1050. PubMed
  22. Vanoli F, et al. 2023. Oncogenesis. 12:8. PubMed
  23. Lei K, et al. 2023. iScience. 26:106001. PubMed
  24. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  25. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  26. Perez GI, et al. 2023. Vaccines (Basel). 11:. PubMed
  27. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  28. Potez M, et al. 2023. Front Oncol. 13:1124272. PubMed
  29. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  30. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  31. Quintela M, et al. 2023. Br J Cancer. . PubMed
  32. Dhoonmoon A, et al. 2022. Nat Commun. 13:5063. PubMed
  33. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  34. Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
  35. Kim EH, et al. 2020. Elife. 9:00. PubMed
  36. Kitayama J, et al. 2016. PLoS One. 11: 0154542. PubMed
  37. Gedye C, et al. 2016. Sci Rep. 6: 25220. PubMed
  38. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  39. Bouhaddou M, et al. 2018. PLoS Comput Biol. 14:e1005985. PubMed
  40. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  41. Dowling CM, et al. 2021. Sci Adv. 7:. PubMed
  42. Fujii T, et al. 2015. Proc Natl Acad Sci U S A. 112: 12800 - 12805. PubMed
  43. Cardoso B, et al. 2015. PLoS One. 10: 0143897. PubMed
  44. Alcon C, et al. 2020. Cell Death Dis. 0.898611111. PubMed
  45. Figueiredo T, et al. 2020. Mol Psychiatry. . PubMed
  46. Lopes-Coelho F, et al. 2021. Front Oncol. 11:656229. PubMed
  47. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  48. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  49. Jones PD, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1711. PubMed
  50. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
  51. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  52. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  53. Hassan MIA, et al. 2021. Comput Struct Biotechnol J. 19:880. PubMed
  54. Ramalingam P, et al. 2020. J Exp Med. 217:00:00. PubMed
  55. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  56. Sales–Dias J, et al. 2019. PLoS One. 14:e0217002. PubMed
  57. Santos LC, et al. 2019. Nat Commun. 1.328472222. PubMed
  58. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  59. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  60. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  61. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  62. Macpherson L, et al. 2020. Nature. 577:266. PubMed
  63. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  64. Karavasili C, et al. 2017. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2017.05.061. PubMed
  65. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  66. Noguchi F, et al. 2017. Mol Cancer Ther. 1.316666667. PubMed
  67. Kumar S, et al. 2018. Cancers (Basel). 0.724305556. PubMed
  68. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  69. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  70. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  71. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  72. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  73. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  74. Pfalzgraff A, et al. 2016. Sci Rep. 6:31577. PubMed
  75. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  76. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed
  77. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  78. Chen MS, et al. 2019. Mol Cancer Res. 17:697. PubMed
  79. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  80. Ning X, et al. 2019. Mol Cell. 74:19. PubMed
  81. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  82. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  83. Wang M, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.097. PubMed
  84. Beeravolu N, McKee C, Chaudhry G 2017. J App Toxicol. 10.1002/jat.3469. PubMed
  85. Kim OV, et al. 2022. iScience. 25:104654. PubMed
  86. Parrula M, et al. 2014. Virus Res. 189:206. PubMed
  87. Brigger D, et al. 2015. Cell Death Dis. 6: e1861. PubMed
  88. Zhang J, et al. 2016. Cell Death Dis. 7:e2266. PubMed
  89. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  90. Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed
  91. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  92. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  93. O'Connor MJ, et al. 2021. Oncogene. 40:4872. PubMed
  94. Esteghamat F, et al. 2019. Nat Genet. 51:1233. PubMed
  95. Blümel E, et al. 2020. Oncoimmunology. 9:1751561. PubMed
  96. Sekine C, et al. 2009. Int Immunol. 1.079861111. PubMed
  97. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  98. Schmitz RL, et al. 2021. Cells. 10:. PubMed
  99. Babaei A, et al. 2021. Adv Pharm Bull. 11:361. PubMed
  100. Madala HR, et al. 2020. Cancer Res. 3492:80. PubMed
  101. Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed
  102. Rauzan M, et al. 2017. PLoS One. 12:e0174107. PubMed
  103. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  104. Chiani M, et al. 2017. Artif Cells Nanomed Biotechnol. 10.1080/21691401.2017.1337029. PubMed
  105. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  106. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
  107. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  108. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  109. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  110. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  111. Müller I, et al. 2020. Mol Cell. 970:77. PubMed
  112. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  113. Norkin M, et al. 2021. Cell Rep. 35:109026. PubMed
  114. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  115. Kamiyama N, et al. 2017. Antiviral Res. 10.1016/j.antiviral.2017.08.007. PubMed
  116. Montero–Herradón S, et al. 2017. Organogenesis. 0.675. PubMed
  117. Nunes SC, et al. 2018. Sci Rep. 6.939583333. PubMed
  118. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  119. Eichelmann AK, et al. 2018. Sci Rep. 8:17553. PubMed
  120. Blümel E, et al. 2019. Oncoimmunology. 8:e1641387. PubMed
  121. Salge-Bartels U, et al. 2014. Thromb Res. 133:244. PubMed
  122. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  123. Khatib JB, et al. 2022. Oncotarget. 13:1078. PubMed
  124. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  125. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  126. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  127. Meyer N, et al. 2021. J Clin Med. 10:. PubMed
  128. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  129. Chheda Z, et al. 2016. J Immunol. 197: 2016 - 2026. PubMed
  130. Slone E, et al. 2015. J Leukoc Biol. 98: 791 - 804. PubMed
  131. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  132. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  133. Zervou S, et al. 2017. PLoS One.. 10.1371/journal.pone.0182994. PubMed
  134. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  135. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  136. Vanoli F, et al. 2021. Oncogene. 40:5095. PubMed
  137. Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed
  138. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  139. Pajarillo E, et al. 2020. J Biol Chem. 295:3040. PubMed
  140. Gholamrezaei M, et al. 2021. Iran J Parasitol. 15:475. PubMed
  141. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  142. Rajavel T, et al. 2017. Sci Rep. 2.665277778. PubMed
  143. Grujic M, et al. 2010. J Immunol. 185:1730. PubMed
  144. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
  145. Meyer N, et al. 2021. Int J Mol Sci. 22:. PubMed
  146. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  147. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  148. Lee DH, et al. 2020. Circ Res. 127:1182. PubMed
  149. Alsina-Sanchis E, et al. 2020. Mol Cancer Res. . PubMed
  150. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  151. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  152. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  153. Polak R, et al. 2015. Blood. 126: 2404 - 2414. PubMed
  154. Matsumoto A, et al. 2019. J Extracell Vesicles. 9:1696517. PubMed
  155. Pirali T, et al. 2017. ChemMedChem. 1.570833333. PubMed
  156. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  157. Dhoonmoon A, et al. 2020. Nucleic Acids Res. 48:7252. PubMed
  158. Nastasi C, et al. 2021. Sci Rep. 11:1458. PubMed
  159. Hafez A, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.46. PubMed
  160. Putheti P, et al. 2010. PLoS One. 5:e8706. PubMed
  161. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
  162. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  163. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  164. Shadboorestan A, et al. 2022. Iran J Basic Med Sci. 25:506. PubMed
  165. Huang Y, et al. 2022. Front Cell Dev Biol. 9:837777. PubMed
  166. Babaei A, et al. 2020. Daru. 28:555. PubMed
  167. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  168. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  169. Liu B, et al. 2016. J Biol Chem. 291: 23869 - 23881. PubMed
  170. Lhuillier C, et al. 2015. J Biol Chem. 290: 16797 - 16811. PubMed
  171. Passaro C, et al. 2013. Endocr Relat Cancer. 20:633. PubMed
  172. Mulik R, et al. 2010. Int J Pharm. 398:190. PubMed
  173. Speth C, et al. 2013. J Infect Dis. 207:823. PubMed
  174. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  175. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  176. Ki H, et al. 2017. Biomed Pharmacother. 10.1016/j.biopha.2017.09.118. PubMed
  177. CO L, et al. 2015. Nat Commun. 6: 7739. PubMed
  178. Huang YL, et al. 2020. Elife. 9:00. PubMed
  179. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  180. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  181. Jackson LM, et al. 2021. Nucleic Acids Res. 49:12855. PubMed
  182. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  183. Di Somma S, et al. 2019. Cancers (Basel). 1.70625. PubMed
  184. Lin W, et al. 2014. PLoS One. 9:83160. PubMed
  185. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
  186. Cao H, et al. 2021. Nat Commun. 12:1792. PubMed
  187. Faria M, et al. 2017. PLoS One. 12(2):e0172689. PubMed
  188. Jones P, et al. 2016. J Biol Chem. 291: 16318 - 16327. PubMed
  189. Awojoodu A, et al. 2014. Blood. 124:1941. PubMed
  190. Intemann J, et al. 2014. Cell Signal. 26:1567. PubMed
  191. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  192. Fang P, et al. 2019. Commun Biol. 0.315972222. PubMed
  193. Kumar S, et al. 2019. Int J Cancer. 146:1652. PubMed
  194. Zhao CC, et al. 2018. Oncol Rep. 40:3392. PubMed
  195. Schleicher EM, et al. 2018. Nucleic Acids Res. 46:8908. PubMed
  196. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  197. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  198. Alcon C, et al. 2021. Cells. 10:. PubMed
  199. Bostanabad SY, et al. 2021. Sci Rep. 11:1539. PubMed
  200. Monzen S, et al. 2022. Mol Clin Oncol. 16:19. PubMed
  201. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  202. Clements KE, et al. 2018. Nucleic Acids Res. 46:8898. PubMed
  203. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  204. Paparella AS, et al. 2021. Nat Commun. 12:6285. PubMed
  205. Huang YL, et al. 2021. iScience. 24:103168. PubMed
  206. Zhang Y, et al. 2021. Cell Death Dis. 0.563194444. PubMed
  207. Kang JH, et al. 2020. Cell Death Dis. 0.803472222. PubMed
  208. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  209. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  210. Coutaz M, et al. 2016. Sci Rep. 6:39117. PubMed
  211. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  212. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  213. Chen Y, et al. 2013. J Exp Med. 210:2515. PubMed
  214. Mendès C, et al. 2019. Sci Rep. 9:14107. PubMed
  215. Lopes–Coelho F, et al. 2017. Oncotarget. 8:82803. PubMed
  216. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  217. Thanuthanakhun N, et al. 2017. PLoS One. . 10.1371/journal.pone.0187610. PubMed
  218. Kuntz E, et al. 2017. Nat Med. 10.1038/nm.4399. PubMed
  219. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  220. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  221. Bottardi S, et al. 2020. Exp Hematol. 68:88. PubMed
  222. Kasikara C, et al. 2021. J Clin Invest. 131:. PubMed
  223. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  224. He J, et al. 2017. FASEB J. 31:2893. PubMed
  225. Lyu X, et al. 2019. BMC Genomics. 1.235416667. PubMed
  226. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  227. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  228. Ning N, et al. 2022. Theranostics. 12:5537. PubMed
  229. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  230. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  231. Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed
  232. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  233. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  234. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
  235. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  236. Gill K, et al. 2013. Biochim Biophys Acta. 1830:2763. PubMed
  237. Young MM, et al. 2019. Cell Death Dis. 1.004861111. PubMed
  238. Santos I, et al. 2019. Nutrients. 2.210416667. PubMed
  239. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  240. Zheng M, et al. 2019. Proc Natl Acad Sci U S A. 116:12422. PubMed
  241. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  242. Wang Y, et al. 2022. Front Cardiovasc Med. 9:876087. PubMed
  243. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
RRID
AB_2561291 (BioLegend Cat. No. 640905)
AB_2561292 (BioLegend Cat. No. 640906)
AB_2629519 (BioLegend Cat. No. 640945)

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Gene ID
308 View all products for this Gene ID
UniProt
View information about Annexin V on UniProt.org

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 7    Revision Date: 10/22/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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