FITC anti-human CD138 Syndecan-1 Antibody anti-CD138

Pricing & Availability

Clone: MI15 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B-B4
Isotype: Mouse IgG1, κ
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Product Citations: publications

MI15_FITC_CD138_Antibody_FC_021913 — Human myeloma cell line U266 was stained with CD138 (clone MI15) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).

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356507

25 tests

£60

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356508

100 tests

£155

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A mixture of U266 and XG-1 human myeloma cell lines.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
SB - Community verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138⁺ cell lines.⁴

Additional reported applications for the relevant formats include: immunofluorescent staining¹, Western blotting², immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections³, and spatial biology (IBEX)^5,6.
Additional Product Notes: This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.
RRID: AB_2561882 (BioLegend Cat. No. 356507)
AB_2561882 (BioLegend Cat. No. 356508)

Antigen Details

Structure: 100-200 kD type I integral transmembrane glycoprotein
Distribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells
Function: Adhesion, controls cell morphology, regulates cell growth
Ligand/Receptor: FGFb, collagen, fibronectin
Cell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication.
Gene ID: 6382 View all products for this Gene ID
UniProt: View information about CD138 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?: It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?: Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
Are other fluorophores compatible with IBEX?: Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
The same antibody works in one tissue type but not another. What is happening?: Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
How can I be sure the staining I’m seeing in my tissue is real?: In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD138 (Syndecan-1)	MI15	FC,SB
Purified anti-human CD138 (Syndecan-1)	MI15	FC,ICC,WB,IHC-P,SB
APC anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138 (Syndecan-1)	MI15	FC,SB
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 700 anti-human CD138 (Syndecan-1)	MI15	FC
PE/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 421™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 510™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 605™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 711™ anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)	MI15	FC,SB
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)	MI15	ICC
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)	MI15	FC
APC/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Pacific Blue™ anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-A0055 anti-human CD138 (Syndecan-1)	MI15	PG
Brilliant Violet 785™ anti-human CD138 (Syndecan-1)	MI15	FC
Biotin anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-C0055 anti-human CD138 (Syndecan-1)	MI15	PG
APC/Fire™ 750 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-B0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Cyanine5 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-D0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Fire™ 640 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 500 anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138	MI15	FC
PE/Fire™ 700 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 423 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™)	MI15	FC
GMP FITC anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 650™ anti-human CD138 (Syndecan-1)	MI15	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 01/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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