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FITC anti-human CD235a (Glycophorin A) Antibody

Pricing & Availability
Clone
HI264 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70312
Other Names
PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group, GYPA
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HI264_FITC_061510
Human red blood cells stained with HI264 FITC
  • HI264_FITC_061510
    Human red blood cells stained with HI264 FITC
Compare all formats See FITC spectral data
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349103 25 µg £70
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349104 100 µg £157
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Description

CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations
  1. Qin K, et al. 2022. Nat Genet. 54:874. PubMed
  2. Perik-Zavodskii R, et al. 2022. Genes (Basel). 13: . PubMed
  3. Abe I, et al. 2022. Dev Cell. 57:2623. PubMed
  4. Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed
  5. Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed
  6. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  7. Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed
  8. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  9. Wu Y, et al. 2019. Nat Med. 25:776. PubMed
  10. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  11. Lan X, et al. 2020. Molecular Cell. 81(2):239-254.e8. PubMed
  12. Heatlie J, et al. 2020. Biopreserv Biobank. 1.070833333. PubMed
  13. Ruiz JP, et al. 2019. Stem Cell Res. 41:101600. PubMed
  14. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  15. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  16. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  17. Martin RM et al. 2019. Cell Stem Cell. 24(5):821-828 . PubMed
  18. Lundin V, et al. 2020. Dev Cell. 446:52. PubMed
  19. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  20. Grajcarek J, et al. 2019. Nat Commun. 3.788888889. PubMed
  21. Weiss R, et al. 2021. Cells. 10:. PubMed
  22. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  23. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  24. Perik-Zavodskii R, et al. 2022. Genes (Basel). 13:. PubMed
RRID
AB_10613463 (BioLegend Cat. No. 349103)
AB_10613463 (BioLegend Cat. No. 349104)

Antigen Details

Structure
Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
Distribution

Erythrocytes and erythroid precursors

Function
Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Ligand/Receptor
Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type
Erythrocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.

Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235a on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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