FITC anti-human CD80 Antibody, CD80, W17149D

Pricing & Availability

Clone: W17149D (See other available formats)
Regulatory Status: RUO
Other Names: B7-1, B7, BB1
Isotype: Rat IgG2a, κ
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Product Citations: publications

W17149D_FITC_CD80_Antibody_030221 — Raji cell line was stained with anti-human CD80 (clone W17149D) FITC (filled histogram) or rat IgG2a, κ FITC isotype control (open histogram).

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375405

25 tests

£53

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375406

100 tests

£126

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Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: RBL1 cells stably transfected with human CD80 (full length)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2890818 (BioLegend Cat. No. 375405)
AB_2890818 (BioLegend Cat. No. 375406)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Lim TS, et al. 2012. PLoS One. 7(9): e45185.
2. Kamphorst, Alice O, et al. 2017. Science. 31;355(6332):1423-1427.
3. Zheng Y, et al. 2004. J. Immunol. 1;172(5):2778-84.
Gene ID: 941 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD80 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD80 Antibody	W17149D	FC
APC anti-human CD80 Antibody	W17149D	FC
FITC anti-human CD80 Antibody	W17149D	FC
PE/Cyanine7 anti-human CD80	W17149D	FC
PE anti-human CD80	W17149D	FC
PerCP/Cyanine5.5 anti-human CD80	W17149D	FC
PE/Fire™ 744 anti-human CD80	W17149D	FC
Spark Blue™ 574 anti-human CD80 (Flexi-Fluor™)	W17149D	FC
Spark Blue™ 550 anti-human CD80 (Flexi-Fluor™)	W17149D	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/02/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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