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FITC anti-human IL-1β Antibody

Pricing & Availability
Clone
JK1B-1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_JK1B-1_FITC_100809
  • A_JK1B-1_FITC_100809
  • B_JK1B-1_FITC_100809
    LP-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 APC then intracellular stained with JK1B-1 FITC (top) or mIgG1,k isotype control FITC (bottom)
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508206 100 tests £161
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-1 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-1 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-1 antibody can neutralize the bioactivity of natural or recombinant IL-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-1 beta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA and ELISPOT Capture: The purified JK1B-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JK1B-2 antibody (Cat. No. 508301) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry: The fluorochrome-labeled JK1B-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1β-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.

Application References

(PubMed link indicates BioLegend citation)
  1. Lu Y, et al. 2015. PNAS. 112:607. PubMed
Product Citations
  1. Rizzo MD, et al. 2019. J Pharmacol Exp Ther. 371:191. PubMed
  2. Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed
  3. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  4. Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
  5. Kaya-Tilki E, et al. 2021. PLoS One. 16:e0260633. PubMed
RRID
AB_345362 (BioLegend Cat. No. 508206)

Antigen Details

Structure
Cytokine; 17.3 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets
B cells, T cells, monocytes
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Does this clone recognize the pro or mature form of IL-1 beta?

The monoclonal anti-human IL-1b antibody clone H1b-98 was generated and screened using the mature form of IL-1b as the immunogen. Since the pro-form contains the mature sequence, it is believed that the antibody also recognize pro-IL-1beta. Similarly, we believe clone JK1B-1 should also recognize both pro and mature forms of IL-1b. However, this information has not been confirmed with in-house testing data.

Go To Top Version: 3    Revision Date: 12/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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