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FITC anti-human Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56)

Pricing & Availability
Clone
UCHT1; HCD14; 3G8; HIB19; 2H7; HCD56;
Regulatory Status
RUO
Other Names
Lin 1
Isotype
Mouse IgG1, κ/Mouse IgG2b, κ
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Product Citations
publications
hlincocktail_fitc_1_062310
Human peripheral blood leukocytes co-stained with CD123 PE and HLA-DR PerCP (gated on the Lineage negative/dim population)
  • hlincocktail_fitc_1_062310
    Human peripheral blood leukocytes co-stained with CD123 PE and HLA-DR PerCP (gated on the Lineage negative/dim population)
  • hlincocktail_fitc_2_062310
    Human peripheral blood leukocytes co-stained with CD123 PE and HLA-DR PerCP (gated on the Lineage negative/dim population)
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348801 50 tests £438
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Description

This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC)
  2. Doherty TA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:577. PubMed.
Product Citations
  1. Shafiei–Jahani P, et al. 2020. Nat Commun. 3.734722222. PubMed
  2. Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed
  3. van der Sluis RM, et al. 2022. EMBO J. 41:e109622. PubMed
  4. Doherty T, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:577. PubMed
  5. Laustsen A, et al. 2021. Elife. 10:. PubMed
  6. Lal A, et al. 2021. Nat Commun. 1507:12. PubMed
  7. Imai Y, et al. 2021. JID Innov. 1:100003. PubMed
  8. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  9. Delaney C, et al. 2021. EMBO Mol Med. 13:e12889. PubMed
  10. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  11. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  12. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  13. Levy Y, et al. 2021. iScience. 24:102711. PubMed
  14. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  15. Zhang JA, et al. 2020. Front Immunol. 11:518. PubMed
  16. Feldman S, et al. 2017. Clin Exp Allergy. 47:457. PubMed
  17. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
  18. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  19. Shevchenko JA, et al. 2020. Oncol Rep. 43:671. PubMed
  20. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
RRID
AB_10612570 (BioLegend Cat. No. 348801)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, neutrophils

Cell Type
B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Gene ID
915 View all products for this Gene ID 2214 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 4684 View all products for this Gene ID 929 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org
Go To Top Version: 2    Revision Date: 07/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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