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FITC anti-human MPO Flow Kit

Pricing & Availability
Clone
MPO421-8B2 (See other available formats)
Regulatory Status
RUO
Other Names
Myeloperoxidase
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_MPO4218B2_FITC_020210
Human peripheral blood intracellularly stained using the myeloperoxidase antibody (top) or isotype control (bottom) included in the BioLegend MPO FITC Flow Kit.
  • A_MPO4218B2_FITC_020210
    Human peripheral blood intracellularly stained using the myeloperoxidase antibody (top) or isotype control (bottom) included in the BioLegend MPO FITC Flow Kit.
  • B_MPO4218B2_FITC_020210
See FITC spectral data
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347201 50 tests £201
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Description

Myeloperoxidase (MPO) is a heterotetrameric protein consisting of two 60 kD heavy units and two 12 kD light units. A lysosomal enzyme, MPO is able to catalyze the production of hypochlorous acid, a potent microbicidal agent, from hydrogen peroxide and chloride anion during the neutrophil respiratory burst. MPO is a major enzyme involved in the inflammatory responses of polymorphonuclear leucocytes. MPO is localized to the azurophilic granules of mature granulocytes and monocytes and is also expressed in some acute myeloid leukemia cells.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  1. Anti-human MPO FITC, Part No. 78079, 50 tests
  2. Mouse IgG1 isotype control FITC, Part No. 78080, 50 tests
  3. MPO Fixation Buffer, Part No. 78078, 50 ml (25 ml x 2 bottles)
  4. Intracellular Staining Perm Wash Buffer, Cat. No. 421002, 100 ml

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
This kit should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells in 100 µl staining volume or 20 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

MPO Staining Procedure:
1. Prepare cells of interest, perform cell surface antigen staining as described in BioLegend’s Cell Surface Immunofluorescence Staining Protocol, then fix cells in 1.0 ml/tube Fixation Buffer (Part No.78078) in the dark for 20 minutes at room temperature.
2. Centrifuge at 350 x g for 5 minutes, discard supernatant.
3. Dilute 10X Intracellular Staining Perm Wash Buffer (Cat. No. 421002) to 1X in DI water.
4. Resuspend fixed cells in 1.0 ml/tube Intracellular Staining Perm Wash Buffer and centrifuge at 350 x g for 5-10 minutes.
5. Repeat step 4 twice.
6. Resuspend fixed/permeabilized cells in the approximate 50-100 µl residual Intracellular Staining Perm Wash Buffer and add 20 µl FITC-conjugated MPO antibody (Part No.78079) or mouse IgG1, κ isotype control (Part No.78080). Incubate for 30 minutes in the dark at room temperature.
7. Wash 2x with 1.0 ml of Intracellular Staining Perm Wash Buffer and centrifuge at 350 x g for 5 minutes.
8. Resuspend the cells in 0.5 ml Cell Staining Buffer and interrogate by flow cytometry using proper machine settings.

Product Citations
  1. Locke M, et al. 2020. Sci Rep. 10:11694. PubMed
  2. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  3. Carestia A, et al. 2016. J Leukoc Biol. 99: 153 - 162. PubMed
  4. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  5. Carestia A, et al. 2016. PLoS One. 11:e0168647. PubMed
  6. Vogelgesang A, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0914-6. PubMed
RRID
AB_2146472 (BioLegend Cat. No. 347201)

Antigen Details

Structure
MPO is a tetrameric protein, each tetramer contains a two 60 kD heavy units and two 12 kD light units. It is a lysosomal hemoprotein stored in azurophilic granules.
Distribution

Neutrophils, monocytes, and some acute myeloid leukemia cells

Cell Type
Leukemia, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Enzymes and Regulators
Antigen References

1. Goedken M, et al. 2007. J. Biol. Chem. 282:27994
2. Nauseef WM, et al. 1988. Euro. J. Haemat. 40:97
3. Kelebanoff SJ, et al.1999. Proc. Assoc. Am. Phys. 111:383

Gene ID
4353 View all products for this Gene ID
UniProt
View information about MPO on UniProt.org
Go To Top Version: 5    Revision Date: 09/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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