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FITC anti-mouse CD172a (SIRPα) Antibody

Pricing & Availability
Clone
P84 (See other available formats)
Regulatory Status
RUO
Other Names
SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
P84_FITC_1_051012
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD172a (clone P84) FITC (top) or rat IgG1, κ FITC isotype control (bottom). Data shown was gated on the myeloid cell population.
  • P84_FITC_1_051012
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD172a (clone P84) FITC (top) or rat IgG1, κ FITC isotype control (bottom). Data shown was gated on the myeloid cell population.
  • P84_FITC_2_051012
  • P84_FITC_3_050319
    Fixed whole mount mouse spleen was stained with FITC CD172a (red) (clone P84), Alexa Fluor® 647 CD11c (yellow) (clone N418), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
Compare all formats See FITC spectral data
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144005 25 µg £70
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144006 100 µg £139
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Description

CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse brain membrane protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
  2. Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
  3. Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
  4. Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sanchez-Molina P, et al. 2022. Brain Behav Immun. 101:231. PubMed
  2. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  3. Ma X, et al. 2023. Nat Commun. 14:1606. PubMed
  4. Ding X, et al. 2021. Nat Commun. 12:2030. PubMed
  5. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  6. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  7. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  8. Marangoni F, et al. 2021. Cell. . PubMed
  9. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  10. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  11. Gozgit JM, et al. 2021. Cancer Cell. :. PubMed
  12. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  13. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
RRID
AB_11204425 (BioLegend Cat. No. 144005)
AB_11204425 (BioLegend Cat. No. 144006)

Antigen Details

Structure
Type I transmembrane protein
Distribution

Monocytes, macrophages, myeloid cells, neuronal tissue

Function
Negative regulation of several biological processes
Interaction
PTPN6, PTPN11
Ligand/Receptor
CD47, SP-A, SP-D
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References

1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510.

Gene ID
19261 View all products for this Gene ID
UniProt
View information about CD172alpha on UniProt.org
Go To Top Version: 3    Revision Date: 10/25/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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