FITC anti-mouse CD45 antibody, CD45, S18009F

Pricing & Availability

Clone: S18009F (See other available formats)
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with anti-mouse CD45 (clone S18009F) FITC (filled histogram) or FITC rat IgG2b, κ isotype control (open histogram).

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157213

25 µg

£21

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157214

100 µg

£53

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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD45
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2894427 (BioLegend Cat. No. 157213)
AB_2894427 (BioLegend Cat. No. 157214)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Interaction: Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.

Trowbridge IS, et al. 1994. Annu. Rev. Immunol. 12:85.

Kishihara K, et al. 1993. Cell. 74:143.

Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

B-Cell Receptor Pathway

Related FAQs

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD45	S18009F	FC
APC/Cyanine7 anti-mouse CD45	S18009F	FC
PE/Cyanine7 anti-mouse CD45	S18009F	FC
PerCP/Cyanine5.5 anti-mouse CD45	S18009F	FC
Alexa Fluor® 700 anti-mouse CD45	S18009F	FC
Pacific Blue™ anti-mouse CD45	S18009F	FC
PE/Fire™ 810 anti-mouse CD45	S18009F	FC
FITC anti-mouse CD45	S18009F	FC
Brilliant Violet 605™ anti-mouse CD45	S18009F	FC
Brilliant Violet 510™ anti-mouse CD45	S18009F	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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