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FITC anti-mouse CD53 Antibody

Pricing & Availability
Clone
OX-79 (See other available formats)
Regulatory Status
RUO
Other Names
OX-79
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
A_OX-79_FITC_102309
C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) PE
  • A_OX-79_FITC_102309
    C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) PE
  • B_OX-79_FITC_102309
    C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
124705 50 µg £77
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124706 200 µg £221
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Description

CD53 is a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). CD53 is highly conserved in evolution, mouse CD53 was 91% identical to rat and 82% identical to human CD53. Mouse CD53 has a molecular mass of 35-45 kD and expresses on virtually all peripheral leukocytes, immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets. CD53 is proposed to play an important role in thymopoiesis and leukocyte signal transduction. It was reported that CD53 interact with a number of proteins, including CD2, CD9, CD19, and CD21. Cross-linking of CD53 promotes B cell activation. It was found that there is a strong correlation between CD53 expression and positive selection of thymocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BAB/14 mouse macrophage cell line RAW264
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Puls KL, et al. 2002. Int. Immunol. 14(3):249.
RRID
AB_2075828 (BioLegend Cat. No. 124705)
AB_2075828 (BioLegend Cat. No. 124706)

Antigen Details

Structure
A 35-42 kD member of the tetraspanin/transmambrane-4 superfamily.
Distribution
Expressed on peripheral leukocytes, some immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets.
Function
Play an important role in leukocyte signal transduction, thymopoiesis, and positive selection of thymocytes.
Interaction
Interact with CD2, CD9, CD19, and CD21.
Cell Type
Leukocytes, Thymocytes, B cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Camo AM, et al. 1995. Eur. J. Immunol. 25(7):2090
2. Tomlinson MG, et al. 1995. Eur. J. Immunol. 25(8):2201
3. Angelisova P, et al. 1994. Immunogenetics. 39(4):249
4. Wright MD, et al. 1993. Int. Immunol. 5(2):209
5. Rasmussen A, et al. 1994. J. Immunol. 153:4997

Gene ID
12508 View all products for this Gene ID
UniProt
View information about CD53 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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