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FITC anti-mouse Ly-6D Antibody

Pricing & Availability
Clone
49-H4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6D, Thymocyte B-cell antigen, ThB
Isotype
Rat IgG2c, κ
Ave. Rating
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Product Citations
publications
49-H4_FITC_1_030812
C57BL6 mouse splenocytes were stained with CD19 APC and Ly-6D (clone 49-H4) FITC (top) or rat IgG2c, κ APC isotype control (bottom).
  • 49-H4_FITC_1_030812
    C57BL6 mouse splenocytes were stained with CD19 APC and Ly-6D (clone 49-H4) FITC (top) or rat IgG2c, κ APC isotype control (bottom).
  • 49-H4_FITC_2_030812
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Cat # Size Price Quantity Check Availability Save
138605 25 µg £61
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138606 100 µg £178
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Description

Lymphocyte antigen 6D (Ly-6D), also known as thymocyte B-cell antigen (ThB), is a 16 kD GPI-linked cell surface glycoprotein. It belongs to the Ly6 gene family and contains one urokinase receptor (uPAR) /Ly6 domain. Ly-6D is involved in cell-cell adhesion and signal transduction. Ly-6D marks the earliest stage of B cell lineage commitment from the common lymphoid progenitors (CLP). It is expressed on B cells, immature thymocytes, and plasmacytoid dendritic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse plasmacytoma MOPC-104E
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Eckhardt LA and Herzenberg LA. 1980. Immunogenetics 11:275. (FC)
Product Citations
  1. Haensel D, et al. 2022. Nat Commun. 13:7520. PubMed
  2. Weiner AI 2022. Cell Reports. 41(11):111805. PubMed
  3. Haensel D, et al. 2023. Nat Commun. 14:2685. PubMed
  4. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  5. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  6. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
RRID
AB_11203888 (BioLegend Cat. No. 138605)
AB_11203888 (BioLegend Cat. No. 138606)

Antigen Details

Structure
GPI-linked cell-surface glycoprotein, molecular weight 16 kD, belongs to the Ly6 gene family, contains one urokinase receptor (uPAR) /Ly6 domain
Distribution

B cells, B cell-biased lymphoid progenitor (BLP), thymocytes, plasmacytoid dendritic cells

Function
Cell-cell adhesion and signal transduction
Cell Type
B cells, Dendritic cells, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules
Antigen References

1. Inlay MA, et al. 2009. Genes Dev. 23:2376.
2. Gumley TP, et al. 1995. Immunol. Cell Biol. 73:277.
3. Mansson R, et al. 2010. Blood 115:2601.
4. Reese JT, et al. 2001. Dev. Immunol. 8:107.

Gene ID
17068 View all products for this Gene ID
UniProt
View information about Ly-6D on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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