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Glial Cell Marker Antibody Sampler Kit

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RUO
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Please refer to individual product datasheets.
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Glial_Cell_Marker_Antbody_Sampler_kit_1_060618
IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_1_060618
    IHC staining of purified anti-P2RY12 antibody (clone S16007D) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve‐All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_2_060618
    IHC staining of anti-CX3CR1 antibody (clone 8E10.D9) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate Heat Induced Epitope Retrieval (H.I.E.R.), the tissue was incubated with clone 8E10.D9 at 20 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection following by hematoxylin and bluing solution counterstain.
  • Glial_Cell_Marker_Antbody_Sampler_kit_3_060618
    IHC staining of purified anti-GFAP antibody (clone SMI 24) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_4_060618
    IHC staining of purified anti-Myelin CNPase antibody (clone SMI 91) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 10X (Cat. No. 927801), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Glial_Cell_Marker_Antbody_Sampler_kit_5_060618
    Western blot of purified anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Glial_Cell_Marker_Antbody_Sampler_kit_6_060618
    Western blot of anti-Myelin Basic Protein antibody (clone P82H9) and isotype-matched IgG1 control. Blots were incubated with anti-MBP at 1 µg/ml (left) or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. MW: molecular weight marker; human brain lysates: 20 µg; rat brain lysates: 20 µg.
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899904 1 kit £306
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Description

The Glial Cell Marker Antibody Sampler Kit offers flexibility for sampling and detection of specific cell types in the central nervous system.  Antibodies included in this kit target specific markers that are used for assessment of protein expression or identification of microglia, astrocytes and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Format Clone Size Reactivity Isotype
Anti-P2RY12 Purified S16007D 25 μg Mouse Rat IgG2b, κ
Anti-CX3CR1 Purified 8E10.D9 25 μg Human Mouse IgG1, λ
Anti-GFAP Purified SMI 24 25 μg Human, Mouse, Rat Mouse IgG2b, κ
Anti-Myelin CNPase Purified SMI 91 25 μg Human, Rat, Mouse Mouse IgG1, κ
Anti-Myelin Basic Protein Purified P82H9 25 μg Human, Rat Mouse IgG1, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

IHC-P, WB - Quality tested
Recommended Usage

Each lot of antibodies in this kit is quality control tested by immunohistochemical staining on formalin-fixed paraffin-embedded tissue or Western blotting. For immunohistochemistry, the suggested uses of these reagents are as follows:

Anti-P2RY12: 5.0 - 10 µg/ml
Anti-CX3CR1: 10 - 20 µg/ml
Anti-GFAP: 1.0 - 5.0 µg/ml
Anti-Myelin CNPase: 0.1 - 1.0 µg/ml

For Western blotting, the suggested uses of these reagents are as follows:

Anti-Myelin Basic Protein: 0.1 - 1.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For verified or reported applications for these antibodies, please see individual product datasheets.

RRID
AB_2734654 (BioLegend Cat. No. 899904)
Go To Top Version: 1    Revision Date: 06/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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