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GMP APC anti-human CD1a Antibody

Pricing & Availability
Clone
SK9 (See other available formats)
Other Names
T6, R4, Leu-6
Isotype
Mouse IgG2b, κ
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Product Citations
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SK9_GMP_APC_CD1a_Antibody_111523
Typical results from human T-cell lymphoblastic leukemia cell line Molt-4 stained either with SK9 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • SK9_GMP_APC_CD1a_Antibody_111523
    Typical results from human T-cell lymphoblastic leukemia cell line Molt-4 stained either with SK9 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
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260348 100 tests £238
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Description

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.

Product Details
Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
400 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for the relevant formats) includes: immunoprecipitation and immunohistochemical staining of frozen sections.2

Application References

(PubMed link indicates BioLegend citation)
  1. Wood GS, et al. 1983. J. Immunol. 131:212.
  2. Facy V, et al. 2005. Toxicol in Vitro. 6:787-95.
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Distribution

Cortical thymocytes, Langerhans cells, dendritic cells

Function
Antigen presentation, lymphocyte activation, thymic T cell development
Ligand/Receptor
CD1-restricted TCRs
Cell Type
Dendritic cells, Langerhans cells, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Wood GS, et al. 1983. J. Immunol. 131:212.
2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
3. Martin LH, et al. 1987. P. Natl. Acad. Sci. USA 84:9189.

Gene ID
909 View all products for this Gene ID
UniProt
View information about CD1a on UniProt.org
Go To Top Version: 1    Revision Date: 12/13/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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