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GMP APC anti-human CD23 Antibody

Pricing & Availability
Clone
EBVCS-5 (See other available formats)
Other Names
Leu-20, FcεRII, IgE Fc Receptor, BLAST-2, B6, Low affinity IgE receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
EBVCS-5_GMP_APC_CD23_081423
Typical results from human peripheral blood lymphocytes stained either with EBVCS APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • EBVCS-5_GMP_APC_CD23_081423
    Typical results from human peripheral blood lymphocytes stained either with EBVCS APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP APC spectral data
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260300 100 tests £238
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Description

CD23 is a 45 kD protein, also known as Leu-20, FcεRII, IgE Fc receptor, BLAST-2, B6, and low affinity IgE receptor. It is a member of the Ig family, expressed on most mature B cells, B cells in follicular mantle (but not in proliferating germinal center cells, follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 responds to high levels of IgE by downregulating IgE secretion. In human monocytes, CD23 triggering results in release of pro-inflammatory cytokines including TNF-α, IL-1, IL-6, and GM-CSF. CD23 can be proteolytically cleaved to generate soluble CD23 fragments of various molecular weights. In chronic lymphocytic leukemia, levels of soluble CD23 in the serum can be used as a prognostic marker to identify patients at high risk for disease progression. Alternate splicing of exon 2 can also generate two cell-surface isoforms of CD23 differing by 6 amino acids in their cytoplasmic region.

Product Details
Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
100 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sugden B and Metzenberg S. 1983. J. Virol. 46:800-807.
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Ig family member, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells, monocytes, eosinophils, a subset of T cells, Langerhans cells

Function
Regulation of IgE synthesis in human monocytes; CD23 triggering results in release of pro-inflammatory cytokines including TNF-a, IL-1, IL-6, and GM-CSF
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells, Eosinophils, Langerhans cells, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Ludin C, et al. 1987. EMBO J. 6:109.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
4. Armant M, et al. 1994. J. Exp. Med. 180:1005.

Gene ID
2208 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org
Go To Top Version: 1    Revision Date: 09/07/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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