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GMP APC/Fire™ 750 anti-human CD4 Antibody

Pricing & Availability
Clone
SK3 (See other available formats)
Other Names
T4, Leu3a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK3_GMP_APCFire750_CD4_Antibody_060123
Typical results from human peripheral blood lymphocytes stained either with SK3 APC/Fire™ 750 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • SK3_GMP_APCFire750_CD4_Antibody_060123
    Typical results from human peripheral blood lymphocytes stained either with SK3 APC/Fire™ 750 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See GMP APC/Fire™ 750 spectral data
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260266 100 tests £245
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
100 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Evans RL, et al. 1981. Immunol. 78:544
  2. Arno A et al. 1999. J. Infect. Dis. 180:56
  3. Muech M, et al. 1997. Blood 89:1364
  4. Wang L, et al. 2012. Cytometry A. 81:567. PubMed
Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PerCP anti-human CD4 SK3 FC
Purified anti-human CD4 SK3 FC,IP
FITC anti-human CD4 SK3 FC
PE anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Biotin anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
APC/Cyanine7 anti-human CD4 SK3 FC
Alexa Fluor® 488 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Alexa Fluor® 700 anti-human CD4 SK3 FC
Purified anti-human CD4 (Maxpar® Ready) SK3 FC,CyTOF®
PE anti-human CD4 SK3 FC
Brilliant Violet 421™ anti-human CD4 SK3 FC
Brilliant Violet 510™ anti-human CD4 SK3 FC
Alexa Fluor® 647 anti-human CD4 SK3 FC
FITC anti-human CD4 SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
PE/Dazzle™ 594 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Brilliant Violet 785™ anti-human CD4 SK3 FC
Brilliant Violet 750™ anti-human CD4 SK3 FC
Brilliant Violet 605™ anti-human CD4 SK3 FC
Brilliant Violet 711™ anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
TotalSeq™-A0045 anti-human CD4 SK3 PG
TotalSeq™-C0045 anti-human CD4 SK3 PG
PE/Cyanine5 anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Spark Blue™ 550 anti-human CD4 SK3 FC
Spark NIR™ 685 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
KIRAVIA Blue 520™ anti-human CD4 SK3 FC
APC/Fire™ 810 anti-human CD4 SK3 FC
PE/Fire™ 640 anti-human CD4 SK3 FC
PE/Fire™ 700 anti-human CD4 SK3 FC
PerCP anti-human CD4 SK3 FC
Spark Violet™ 538 anti-human CD4 Antibody SK3 FC
Spark YG™ 581 anti-human CD4 SK3 FC
Alexa Fluor® 660 anti-human CD4 SK3 FC
Spark YG™ 593 anti-human CD4 SK3 FC
PE/Fire™ 810 anti-human CD4 Antibody SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Blue™ 574 anti-human CD4 SK3 FC
GMP APC anti-human CD4 SK3 FC
TotalSeq™-B0045 anti-human CD4 Antibody SK3 PG
Spark Violet™ 423 anti-human CD4 Antibody SK3 FC
GMP Pacific Blue™ anti-human CD4 SK3 FC
Spark UV™ 387 anti-human CD4 SK3 FC
GMP PE anti-human CD4 SK3 FC
GMP FITC anti-human CD4 SK3 FC
Spark Red™ 718 anti-human CD4 SK3 FC
GMP PerCP anti-human CD4 SK3 FC
GMP PE/Cyanine7 anti-human CD4 SK3 FC
Spark Violet™ 500 anti-human CD4 SK3 FC
GMP PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Brilliant Violet 650™ anti-human CD4 SK3 FC
PerCP/Fire™ 806 anti-human CD4 SK3 FC
Spark Blue™ 515 anti-human CD4 SK3 FC
GMP APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Violet™ 423 anti-human CD4 SK3 FC
PerCP/Fire™ 780 anti-human CD4 SK3 FC
PE/Fire™ 744 anti-human CD4 SK3 FC
Spark PLUS UV395™ anti-human CD4 SK3 FC
Spark PLUS B550™ anti-human CD4 SK3 FC
Go To Top Version: 1    Revision Date: 06/19/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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