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GMP Ultra-LEAF™ Purified anti-human CD28 SF Antibody

Pricing & Availability
Clone
S20013F (See other available formats)
Other Names
T44, Tp44
Isotype
Mouse IgG1, κ
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S20013F_GMP_ULEAF_CD28_Antibody_1_012623
Human peripheral blood lymphocytes were stained with GMP Ultra-LEAF™ anti-human CD28 SF (Clone S20013F, filled histogram) or isotype control (Clone MG1-45, open histogram) followed by anti-mouse IgG FITC stain.
  • S20013F_GMP_ULEAF_CD28_Antibody_1_012623
    Human peripheral blood lymphocytes were stained with GMP Ultra-LEAF™ anti-human CD28 SF (Clone S20013F, filled histogram) or isotype control (Clone MG1-45, open histogram) followed by anti-mouse IgG FITC stain.
  • S20013F_GMP_ULEAF_CD28_Antibody_2_012623
    PBMC-derived T cells were activated in the presence of GMP Ultra-LEAF™ anti-human CD3 antibody (Cat. No. 317354) alone or combined with Ultra-LEAF™ anti-human CD28 (Cat. No. 377604) or GMP Ultra-LEAF™ anti-human CD28 SF antibody (Cat. No. 377606). Activation was done via plate coating with 1 µg/mL of each antibody. Cells were cultured for a total of 11 days and viable cell number was determined.
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377605 100 µg £264
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377606 1 mg £1355
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Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD28 transfected cells
Formulation
0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Costim - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. S20013F antibody can be used at 1 μg/mL of antibody in PBS in combination with 1 μg/mL of anti-human CD3 antibody for T cell activation. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S20013F antibody can completely block the binding of clone CD28.2 on target.

Clone S20013F can partially block Clone S20013B on target.

Clone S20013F can increase anti-CD3 induced human peripheral T-cell proliferation.

Additional Product Notes

This antibody is intended for activation and expansion of T cells for research and ex vivo cell culture applications. The antibody was GMP manufactured under serum-free conditions, including serum-free hybridoma cell culture, without additional animal or human-derived materials.

 

For plate coating protocols, S20013F antibody (Cat. No. 377605/377606) can be used at 1 μg/mL in PBS in combination with 1 μg/mL of anti-human CD3 antibody (Cat. No. 317354) to efficiently activate T cells. Plates should be coated for 2 hours in a tissue culture incubator or overnight at 4°C. For soluble antibody activation protocols, we recommend 10 μg/mL of anti-human CD3 (Cat. No. 317354) antibody and 5 μg/mL of anti-human CD28 antibody (Cat. No. 377605).

Disclaimer

GMP Ultra-LEAF™ antibodies. BioLegend GMP Ultra-LEAF™ antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency    
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12

 

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBVpositive B cells

Function
T cell costimulation
Ligand/Receptor
CD80/CD86
Cell Type
Lymphocytes, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Cell Proliferation and Viability, Costimulatory Molecules, Immuno-Oncology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
  2. June CH, et al. 1994. Immunol Today. 15:321-31.
  3. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212
Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org
Go To Top Version: 2    Revision Date: 03/16/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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