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Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) Antibody

Pricing & Availability
Clone
A16002B (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16002B_GoChIP_STAT3_Phosph_Antibody_1_091117
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) (Clone A16002B), or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • A16002B_GoChIP_STAT3_Phosph_Antibody_1_091117
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) (Clone A16002B), or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • A16002B_PURE_STAT3_Phospho_Antibody_2_092816
    Total cell lysate from PC3 cells (lane 1, 15 µg), HeLa cells (lane 2, 15 µg), untreated HepG2 (lane 3, 15 µg) and hIL-6 (10 ng/ml, 20 minutes) treated HepG2 (lane 4, 15 µg) were probed with purified anti-STAT3 Phospho (Tyr705) (clone A16002B) antibody and anti-mouse IgG antibody. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • A16002B_PURE_STAT3_Phospho_Antibody_3_092816
    Human whole blood was stimulated with (filled histogram), or without (open histogram), IL-6 for 15 minutes, treated with RBC Lysis/Fixation Solution (10X), permeabilized with True-Phos™ Perm Buffer, and then stained with purified STAT3 Phospho (Tyr705) (clone A16002B), followed by AlexaFluor® 647 goat anti-mouse IgG antibody. Data shown is gated on lymphocytes.
  • A16002B_PURE_STAT3_Phospho_Antibody_3_WB_120117
    HepG2 cells were starved overnight and either left untreated (lane 1) or treated with IL-6 (lane 2). Total cell lysates (15 µg protein) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose membrane, and probed with a 1:10000 (0.05 µg/mL) dilution of purified anti-STAT3 Phospho (Tyr705) antibody (clone A16002B). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated goat anti-mouse-IgG secondary antibody for the anti-STAT3 Phospho (Tyr705) (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder
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690405 25 µg £57
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690406 100 µg £178
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Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT3 peptide phosphorylated at Y705. Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide
Preparation
The Go-ChIP-Grade™ Purified antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by ChIP. For ChIP, the suggested use of this reagent is 1:50 dilution by volume. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Recognition of pan STAT3, weak cross-reactivity with mouse (C57BL/6) STAT3 Phospho.

The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.

This clone recognizes both unphosphorylated and phosphorylated (Tyr705) STAT3 protein.

RRID
AB_2716053 (BioLegend Cat. No. 690405)
AB_2716053 (BioLegend Cat. No. 690406)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous.

Function
STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. The small GTPase Rac1 binds and regulates activity of STAT3.
Interaction
When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho Tyr705 on UniProt.org
Go To Top Version: 0    Revision Date: 09/13/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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