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HRP anti-α-Synuclein, 34-45 Antibody

Pricing & Availability
Clone
A15110D (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15110D_HRP_alpha-synuclein_Antibody_042518
IHC staining of HRP anti-α-Synuclein, 34-45 antibody (clone A15110D) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson's disease (right panel) human brain tissues. Following antigen retrieval using 88% formic acid, the tissues were incubated with 5.0 µg/mL of the primary antibody for 1 hour at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • A15110D_HRP_alpha-synuclein_Antibody_042518
    IHC staining of HRP anti-α-Synuclein, 34-45 antibody (clone A15110D) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson's disease (right panel) human brain tissues. Following antigen retrieval using 88% formic acid, the tissues were incubated with 5.0 µg/mL of the primary antibody for 1 hour at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
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849104 100 µg £174
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Description

α-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including  the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated α-synuclein synthetic peptide that incorporated amino acids 34-45.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

By ELISA, this antibody cross-reacts with ß-synuclein.

RRID
AB_2734625 (BioLegend Cat. No. 849104)

Antigen Details

Structure
α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein, Phospho-Proteins
Antigen References

1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501(7465):45.
3. Bartels T, et al. 2011. Nature. 477(7362): 107.
4. Devine MJ, et al. 2011. Mov. Disord. 26:2160.
5. Mahul-Mellier AL, et al. 2014. Hum. Mol. Genet. 23(11):2858.

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein 34-45 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/26/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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